Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8(T)

The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8(T). This study considered six hydrocarbon mixtures that were tested for 22days at two different cultivation temperatures (4 and 15°C). During the incubation...

Full description

Saved in:
Bibliographic Details
Published in:Marine pollution bulletin 2016-04, Vol.105 (1), p.125-130
Main Authors: Gentile, G, Bonsignore, M, Santisi, S, Catalfamo, M, Giuliano, L, Genovese, L, Yakimov, M M, Denaro, R, Genovese, M, Cappello, S
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
Biodegradation, Environmental
Hydrocarbons - metabolism
Oils
Petroleum - metabolism
Water Pollutants, Chemical - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8(T). This study considered six hydrocarbon mixtures that were tested for 22days at two different cultivation temperatures (4 and 15°C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography-flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4',6-diamidino-2-phenylindole) staining showed a linear increase during the first 18days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15°C rather than 4°C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4°C.
ISSN:1879-3363
DOI:10.1016/j.marpolbul.2016.02.041