Combination of aspartic acid and glutamic acid inhibits tumor cell proliferation

Placental extract contains several biologically active compounds, and pharmacological induction of placental extract has therapeutic effects, such as improving liver function in patients with hepatitis or cirrhosis. Here, we searched for novel molecules with an anti-tumor activity in placental extra...

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Bibliographic Details
Published in:Biomedical Research 2016/04/01, Vol.37(2), pp.153-159
Main Authors: YAMAGUCHI, Yoshie, YAMAMOTO, Katsunori, SATO, Yoshinori, INOUE, Shinjiro, MORINAGA, Tetsuo, HIRANO, Eiichi
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - pharmacology
Aspartic Acid - pharmacology
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Glutamic Acid - pharmacology
Humans
Liver Neoplasms - drug therapy
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Placenta - chemistry
Placenta - metabolism
Pregnancy
Proto-Oncogene Proteins c-akt - metabolism
Rabbits
Signal Transduction - drug effects
Xenograft Model Antitumor Assays
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Summary:Placental extract contains several biologically active compounds, and pharmacological induction of placental extract has therapeutic effects, such as improving liver function in patients with hepatitis or cirrhosis. Here, we searched for novel molecules with an anti-tumor activity in placental extracts. Active molecules were separated by chromatographic analysis, and their antiproliferative activities were determined by a colorimetric assay. We identified aspartic acid and glutamic acid to possess the antiproliferative activity against human hepatoma cells. Furthermore, we showed that the combination of aspartic acid and glutamic acid exhibited enhanced antiproliferative activity, and inhibited Akt phosphorylation. We also examined in vivo tumor inhibition activity using the rabbit VX2 liver tumor model. The treatment mixture (emulsion of the amino acids with Lipiodol) administered by hepatic artery injection inhibited tumor cell growth of the rabbit VX2 liver. These results suggest that the combination of aspartic acid and glutamic acid may be useful for induction of tumor cell death, and has the potential for clinical use as a cancer therapeutic agent.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.37.153