Interleukin-21 expression in hippocampal astrocytes is enhanced following kainic acid-induced seizures

Objective: Interleukin-21 (IL-21) is a cytokine that is an important modulator of immune responses. However, its roles in epilepsy are not completely clear. Here, we investigated the expression and distribution of IL-21 in a kainic acid (KA)-induced acute seizure mouse model. Materials and methods:...

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Bibliographic Details
Published in:Neurological research (New York) 2016-02, Vol.38 (2), p.151-157
Main Authors: Xiong, Xiao-Yi, Wang, Ting-Gang, Yang, Mei-Hua, Meng, Zhao-You, Yang, Qing-Wu, Wang, Fa-Xiang
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Astrocyte
Astrocytes - drug effects
Astrocytes - metabolism
CD11b Antigen - metabolism
Disease Models, Animal
Excitatory Amino Acid Agonists - toxicity
Gene Expression Regulation - drug effects
Glial Fibrillary Acidic Protein - metabolism
Hippocampus - pathology
Inflammatory cytokines
Interleukin-21
Interleukins - genetics
Interleukins - metabolism
Kainic acid
Kainic Acid - toxicity
Mice
Mice, Inbred C57BL
Phosphopyruvate Hydratase - metabolism
RNA, Messenger - metabolism
Seizures
Seizures - chemically induced
Seizures - pathology
Time Factors
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Summary:Objective: Interleukin-21 (IL-21) is a cytokine that is an important modulator of immune responses. However, its roles in epilepsy are not completely clear. Here, we investigated the expression and distribution of IL-21 in a kainic acid (KA)-induced acute seizure mouse model. Materials and methods: Mice (n = 146) were randomly divided into an age-matched group, PBS injection group, and a KA injection group. The KA-injected mice were evaluated at 1, 3, and 24 h post-injection. IL-21 mRNA and protein expression levels were measured using RT-PCR and western blotting. Immunohistochemistry and immunofluorescence staining were performed to further characterize the pattern and distribution of IL-21 expression. Results: The IL-21 mRNA and protein expression levels in the hippocampal tissues of the KA-treated mice were significantly increased as early as 1 h compared with the age-matched mice and PBS-treated mice. After this time point, the expression was reduced, but it remained higher than the level in the PBS-treated mice (p 
ISSN:0161-6412
1743-1328
DOI:10.1080/01616412.2015.1135557