Characterization and in vitro biological evaluation of mineral/osteogenic growth peptide nanocomposites synthesized biomimetically on titanium

•Mineral/OGP nanocomposite layers were synthesized biomimetically on Ti substrates.•Incorporated OGP affected the morphology and ultimate structure of mineral.•Incorporated OGP improved the MSCs adhesion, proliferation, and ALP activity. Nanocomposite layers of mineral/osteogenic growth peptide (OGP...

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Bibliographic Details
Published in:Applied surface science 2015-04, Vol.334, p.62-68
Main Authors: Chen, Cen, Kong, Xiangdong, Zhang, Sheng-Min, Lee, In-Seop
Format: Article
Language:English
Subjects:
Biological
Biomedical materials
Cell adhesion
Coprecipitation
Mineral
Minerals
Morphology
Nanocomposites
Osteogenic growth peptide
Peptides
Titanium
Titanium base alloys
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Summary:•Mineral/OGP nanocomposite layers were synthesized biomimetically on Ti substrates.•Incorporated OGP affected the morphology and ultimate structure of mineral.•Incorporated OGP improved the MSCs adhesion, proliferation, and ALP activity. Nanocomposite layers of mineral/osteogenic growth peptide (OGP) were synthesized on calcium phosphate coated titanium substrates by immersing in calcium-phosphate buffer solution containing OGP. Peptide incorporated mineral was characterized by determining quantity loaded, effects on mineral morphology and structure. Also, the biological activity was investigated by cell adhesion, proliferation assay, and measurement of alkaline phosphatase (ALP) activity. X-ray photoelectron spectroscopy (XPS) and micro-bicinchoninic acid (BCA) assay revealed that OGP was successfully incorporated with mineral and the amount was increased with immersion time. Incorporated OGP changed the mineral morphology from sharp plate-like shape to more rounded one, and the octacalcium phosphate structure of the mineral was gradually transformed into apatite. With confocal microscopy to examine the incorporation of fluorescently labeled peptide, OGP was evenly distributed throughout mineral layers. Mineral/OGP nanocomposites promoted cell adhesion and proliferation, and also increased ALP activity of mesenchymal stem cells (MSCs). Results presented here indicated that the mineral/OGP nanocomposites formed on titanium substrates had the potential for applications in dental implants.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2014.08.041