Detection of plasmid mediated colistin resistance (MCR-1) in Escherichia coli and Salmonella enterica isolated from poultry and swine in Spain

• Published in: Research in veterinary science 2016-04, Vol.105, p.134-135
• Main Authors: Quesada, Alberto, Ugarte-Ruiz, María, Iglesias, M. Rocío, Porrero, M. Concepción, Martínez, Remigio, Florez-Cuadrado, Diego, Campos, María J., García, María, Píriz, Segundo, Sáez, José Luis, Domínguez, Lucas
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Colistin - pharmacology; Colistin resistance; Drug Resistance, Bacterial; E coli; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - physiology; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Feces; Genetic engineering; Lymphatic system; mcr-1; Mutation; Plasmid; Plasmids; PmrAB; Poultry; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; S. enterica; Salmonella; Salmonella enterica; Salmonella enterica - drug effects; Salmonella enterica - physiology; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; Salmonella typhimurium - drug effects; Salmonella typhimurium - physiology; Sequence Analysis, DNA - veterinary; Spain - epidemiology; Swine; Swine Diseases - epidemiology; Swine Diseases - microbiology; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2016.02.003

Recent findings suggest that use of colistin as a last resort antibiotic is seriously threatened by the rise of a new plasmid mediated mechanism of resistance (MCR-1). This work identifies, for the first time in Southern Europe, the gene mcr-1 in nine strains from farm animals (poultry and swine) corresponding to five Escherichia coli and four Salmonella enterica, among which three belong to serovar Typhimurium and one to Rissen. The MCR-1 was found encoded by a plasmid highly mobilizable by conjugation to the E. coli J53 strain. Two E. coli strains carried two determinants, mcr-1 plus pmrA or pmrB mutations, known to confer colistin resistance. •MCR-1 is detected in E. coli and Salmonella from poultry and swine, in Spain.•The plasmid carrying mcr-1 has approximately 60kb.•Colistin resistance is efficiently mobilized by conjugation from MCR-1 positive strains.