A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms

Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free c...

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Bibliographic Details
Published in:Analytical methods 2016-04, Vol.8 (14), p.288-2886
Main Authors: Buser, J. R, Zhang, X, Byrnes, S. A, Ladd, P. D, Heiniger, E. K, Wheeler, M. D, Bishop, J. D, Englund, J. A, Lutz, B, Weigl, B. H, Yager, P
Format: Article
Language:English
Subjects:
Bacteria
Drying
Enzymes
Heaters
Heating
Heating equipment
Human respiratory syncytial virus
Ribonucleic acids
Staphylococcus aureus
Viruses
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Summary:Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical heater system for rapid lysis of a Gram-positive bacterium ( Staphylococcus aureus ) and an RNA virus (human respiratory syncytial virus) using a dried lysis enzyme mixture (achromopeptidase) for S. aureus . After a lysis step (
ISSN:1759-9660
1759-9679
DOI:10.1039/c6ay00107f