Sporadic Creutzfeldt–Jakob disease diagnostic accuracy is improved by a new CSF ELISA 14-3-3γ assay

Highlights • We quantified CSF 14-3-3γ in 72 sCJD and 73 Non-CJD patients, by a new ELISA assay. • WB and ELISA 14-3-3γ results matched in 81% of all cases. • WB inconclusive cases were further discriminated by ELISA 14-3-3γ assay. • ELISA 14-3-3γ is the best single predictive marker for sCJD early...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience 2016-05, Vol.322, p.398-407
Main Authors: Leitão, M.J, Baldeiras, I, Almeida, M.R, Ribeiro, M.H, Santos, A.C, Ribeiro, M, Tomás, J, Rocha, S, Santana, I, Oliveira, C.R
Format: Article
Language:English
Subjects:
14-3-3 protein
14-3-3 Proteins - cerebrospinal fluid
Adolescent
Adult
Aged
Aged, 80 and over
Apolipoproteins E - genetics
Biomarkers - cerebrospinal fluid
Blotting, Western
Child
Creutzfeldt-Jakob Syndrome - cerebrospinal fluid
Creutzfeldt-Jakob Syndrome - genetics
CSF
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Male
Middle Aged
Neurology
Phosphorylation
Prion Proteins - genetics
sCJD
Sensitivity and Specificity
Tau proteins
tau Proteins - cerebrospinal fluid
Western Blot
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Highlights • We quantified CSF 14-3-3γ in 72 sCJD and 73 Non-CJD patients, by a new ELISA assay. • WB and ELISA 14-3-3γ results matched in 81% of all cases. • WB inconclusive cases were further discriminated by ELISA 14-3-3γ assay. • ELISA 14-3-3γ is the best single predictive marker for sCJD early diagnosis. • PRNP genotype did not influence ELISA 14-3-3γ protein levels.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2016.02.057