Changed Serum Cytokine Profile in Mice in Response to Streptococcus A Culture

• Published in: Bulletin of experimental biology and medicine 2015-05, Vol.159 (1), p.66-69
• Main Authors: Danilova, T. A., Adzhieva, A. A., Danilina, G. A., Lunin, V. G., Grabko, V. I., Minko, A. G.
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antigens; Antigens, Bacterial - immunology; Bacterial Outer Membrane Proteins - immunology; Biomedical and Life Sciences; Biomedicine; Carrier Proteins - immunology; Cell Biology; Cytokines; Cytokines - blood; Epitopes - immunology; Granulocyte-Macrophage Colony-Stimulating Factor - blood; Granulocyte-Macrophage Colony-Stimulating Factor - secretion; Health aspects; Infection; Inflammation; Injections, Intraperitoneal; Interferon-gamma - blood; Interferon-gamma - secretion; Interleukins - blood; Interleukins - secretion; Internal Medicine; Laboratory Medicine; Male; Mice; Mice, Inbred CBA; Molecular Mimicry; Myocardium - immunology; Pathology; Serogroup; Streptococcus; Streptococcus pyogenes - classification; Streptococcus pyogenes - immunology; Th1 Cells - immunology; Th1 Cells - secretion; Th2 Cells - immunology; Th2 Cells - secretion; Time Factors; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - secretion
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2891-z

Comparative analysis of serum cytokine profiles of CBA mice was carried out 1, 5, 24, and 48 h after intraperitoneal injection of killed culture of different streptococcus A types. The production of cytokines in response to different streptococcus types varied. The highest level was recorded in response to types 1M and 3T+M, more often detected in invasive streptococcal infection. The highest levels of IL-2 were recorded in response to 1M (47-fold increase in comparison with the control) and 3T+M streptococcus types (more than 10-fold increase). Injections of these types also led to an increase of IFN-γ level (15.6 and 11.3 times, respectively). The level of TNF-α increased less (3.6 times in response to 3T+M and 2.6 times in response to 1M type). The levels of IL-5, IL-10, and IL-12 increased 2-3-fold. Injections of 1T and 5M types led to just a 2-fold increase of cytokine levels. These data indicated induction of the immune response trend by mainly Th1 or mixed Th1/Th2 pattern in response to group A streptococcus antigens.