Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén)

BACKGROUND: Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance. RESULTS: An imid...

Full description

Saved in:
Bibliographic Details
Published in:Pest management science 2016-05, Vol.72 (5), p.1051-1058
Main Authors: Elzaki, Mohammed Esmail Abdalla, Zhang, Wanfang, Feng, Ai, Qiou, Xiaoyan, Zhao, Wanxue, Han, Zhaojun
Format: Article
Language:English
Subjects:
Animals
China
constitutive overexpression
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
cytochrome P450
Gene expression
Hemiptera - drug effects
Hemiptera - genetics
Hemiptera - metabolism
imidacloprid
Imidazoles - pharmacology
Insect Proteins - genetics
Insect Proteins - metabolism
Insecticide Resistance
Insecticides
Insecticides - pharmacology
Laodelphax striatellus
Neonicotinoids
Nitro Compounds - pharmacology
RNA interference
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND: Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance. RESULTS: An imidacloprid‐resistant strain was produced by selecting a field population with imidacloprid for 24 generations. Piperonyl butoxide (PBO) showed a 1.70‐fold synergistic effect. Enzyme activity assays were conducted, and cytochrome P450 monooxygenase showed 1.88‐fold activity. The mRNA expression levels of 57 P450 genes were compared. Four CYP genes were found to be overexpressed and significantly different to the susceptible strain. Four strains were selected with imidacloprid for a short period, and the expression levels of ten identified detoxification genes were then compared. Only CYP353D1v2 overexpressed and was significantly different to the susceptible strain. Strong correlation was found between CYP353D1v2 expression levels and imidacloprid treatments. Additionally, gene‐silencing RNAi via dsRNA feeding showed that depressing the expression of CYP353D1v2 could significantly enhance the sensitivity of L. striatellus to imidacloprid. CONCLUSION: Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long‐term selection, and expression of CYP353D1v2 with imidacloprid resistance in short‐term selection in L. striatellus. © 2015 Society of Chemical Industry
ISSN:1526-498X
1526-4998
DOI:10.1002/ps.4155