Expression of Immunomodulator Gene mRNA in Co-Culture of Mesenchymal Stromal Cells from the Placenta and Human Mononuclear Blood Cells

• Published in: Bulletin of experimental biology and medicine 2015-02, Vol.158 (4), p.561-565
• Main Authors: Savilova, A. M., Chulkina, M. M., Veryasov, V. N., Gordeeva, M. N., Trofimov, D. Yu, Sukhikh, G. T.
• Format: Article
• Language: English
• Subjects: B cells; Biomedical and Life Sciences; Biomedicine; Cell Biology; DNA Primers - genetics; Female; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Genetic research; Histocompatibility antigens; HLA histocompatibility antigens; Humans; Immunologic Factors - genetics; Immunologic Factors - metabolism; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-2 Receptor alpha Subunit - genetics; Interleukin-2 Receptor alpha Subunit - metabolism; Internal Medicine; Laboratory Medicine; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Mesenchymal Stromal Cells - immunology; Mesenchymal Stromal Cells - metabolism; Messenger RNA; Pathology; Placenta - cytology; Pregnancy; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem cells; T cells; T-Lymphocytes, Regulatory - immunology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2807-y

The expression of mRNA of cytokines and immunoregulatory molecules characterizing the interaction of mesenchymal stromal cells from chorionic villi of postpartum placenta and allogenic mononuclear blood cells was studied during 3-day co-culturing of these cells. The expression of foxp3 , il2ra , and il10 mRNA in floating mononuclear cells increased from day 1 to 3 in co-culture, which can refl ect the process of induction of regulatory T cells in the lymphocyte population under the action of mesenchymal stromal cells.