A 90-day dietary study of a (2R,4R)-monatin salt in Beagle dogs

• Published in: Food and chemical toxicology 2016-05, Vol.91, p.181-190
• Main Authors: Crincoli, Christine M., Brathwaite, Witty A., Rihner, Marisa O., Nikiforov, Andrey I., Harris, Stephen B., Greeley, Melanie A., Eapen, Alex K.
• Format: Article
• Language: English
• Subjects: Animals; Body Weight - drug effects; Diet; Dietary; Dog; Feeding Behavior - drug effects; Female; Glutamic Acid - administration & dosage; Glutamic Acid - analogs & derivatives; Indoles - administration & dosage; Male; Organ Size - drug effects; R,R-monatin; Safety; Toxicity
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2015.08.020

(2R,4R)-Monatin salt (Na/K) [sodium/potassium (2R,4R)-2-amino-4-carboxy-4-hydroxy-5-(3-indolyl) pentanoate, hereafter “R,R-monatin”] was administered in the diets of groups of Beagle dogs (4/sex/group) at concentrations of 0 (basal diet), 5000, 20,000, or 35,000 ppm for 13 weeks. There were no effects on survival, clinical observations, body weight and body weight gain, feed consumption and feed efficiency, functional observational battery, ophthalmic examination, and electrocardiographic evaluation. No adverse effects on hematology, serum chemistry, and urinalysis parameters were reported. A statistically significant decrease in testicular weights associated with germ cell hypocellularity and reduced luminal sperm in the epididymides was reported in all treated male groups. Based on these findings, the dietary no-observed-adverse-effect level (NOAEL) of R,R-monatin for 90 days was considered 35,000 ppm for female dogs (approximately 1101 mg/kg bw/day) and