Molecular Cloning and Characterization of O-Methyltransferase from Mango Fruit (Mangifera indica cv. Alphonso)

Flavour of ripe Alphonso mango is invariably dominated by the de novo appearance of lactones and furanones during ripening. Of these, furanones comprising furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone) are of particular importance due to their...

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Bibliographic Details
Published in:Molecular biotechnology 2016-05, Vol.58 (5), p.340-350
Main Authors: Chidley, Hemangi G., Oak, Pranjali S., Deshpande, Ashish B., Pujari, Keshav H., Giri, Ashok P., Gupta, Vidya S.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biochemistry
Biological Techniques
Biosynthesis
Biotechnology
Cell Biology
Chemistry
Chemistry and Materials Science
Cloning
Cloning, Molecular
DNA, Complementary - genetics
Enzymes
Fruits
Human Genetics
Mangifera - enzymology
Mangifera indica
Methyltransferases - chemistry
Methyltransferases - genetics
Methyltransferases - metabolism
Original Paper
Protein Science
Sequence Homology, Amino Acid
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Summary:Flavour of ripe Alphonso mango is invariably dominated by the de novo appearance of lactones and furanones during ripening. Of these, furanones comprising furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone) are of particular importance due to their sweet, fruity caramel-like flavour characters and low odour detection thresholds. We isolated a 1056 bp complete open reading frame of a cDNA encoding S -adenosyl- l -methionine-dependent O -methyltransferase from Alphonso mango. The recombinantly expressed enzyme, MiO MTS showed substrate specificity towards furaneol and protocatechuic aldehyde synthesizing mesifuran and vanillin, respectively, in an in vitro assay reaction. A semi-quantitative PCR analysis showed fruit-specific expression of MiOMTS transcripts. Quantitative real-time PCR displayed ripening-related expression pattern of MiOMTS in both pulp and skin of Alphonso mango. Also, early and significantly enhanced accumulation of its transcripts was detected in pulp and skin of ethylene-treated fruits. Ripening-related and fruit-specific expression profile of MiOMTS and substrate specificity towards furaneol is a suggestive of its involvement in the synthesis of mesifuran in Alphonso mango. Moreover, a significant trigger in the expression of MiOMTS transcripts in ethylene-treated fruits point towards the transcriptional regulation of mesifuran biosynthesis by ethylene.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-016-9933-2