Microarray analysis of gene expression in proliferating Schwann cells: Synergistic response of a specific subset of genes to the mitogenic action of heregulin plus forskolin

Cultured Schwann cells treated with heregulin growth factor require costimulation with a cyclic adenosine monophosphate‐elevating agent to produce maximal cell proliferation. Gene chip expression analysis was used to identify genes that are induced or repressed when Schwann cells are treated with he...

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Bibliographic Details
Published in:Journal of neuroscience research 2003-08, Vol.73 (4), p.456-464
Main Authors: Schworer, Charles M., Masker, Kathryn K., Wood, G. Craig, Carey, David J.
Format: Article
Language:English
Subjects:
Animals
Cell Division - drug effects
Cell Division - genetics
Cells, Cultured
Colforsin - pharmacology
Cyclins - metabolism
DNA Topoisomerases, Type I - metabolism
Down-Regulation
Drug Synergism
expression profiling
Gene Expression Regulation - drug effects
glia
growth factors
Immunoblotting
Mitosis - drug effects
Mitosis - physiology
Neuregulin-1 - physiology
Oligonucleotide Array Sequence Analysis - methods
Proliferating Cell Nuclear Antigen - metabolism
Rats
RNA - biosynthesis
Schwann Cells - drug effects
Schwann Cells - physiology
Sciatic Nerve
Up-Regulation
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Summary:Cultured Schwann cells treated with heregulin growth factor require costimulation with a cyclic adenosine monophosphate‐elevating agent to produce maximal cell proliferation. Gene chip expression analysis was used to identify genes that are induced or repressed when Schwann cells are treated with heregulin and/or forskolin. By utilizing arrays that contained 8,799 probes, the expression of over 1,000 genes was found to be significantly changed after 30 hr of treatment with heregulin, forskolin, or heregulin plus forskolin. Hierarchical clustering revealed groups of genes with distinct expression patterns. Of particular interest was a cluster of 140 genes that were up‐regulated by heregulin plus forskolin but not by heregulin or forskolin alone. Many of the genes in this group have roles in cell division, such as cyclin B, cyclin D3, E2F‐5, cdc 25B, polo‐like kinase, and protein kinase C type III. These findings identify a profile of gene expression for Schwann cell proliferation. © 2003 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.10679