Epistylis portoalegrensis n. sp. (Ciliophora, Peritrichia): A New Freshwater Ciliate Species from Southern Brazil

• Published in: The Journal of eukaryotic microbiology 2016-01, Vol.63 (1), p.93-99
• Main Authors: Kühner, Steffen, Simão, Taiz L. L, Safi, Lúcia S. L, Gazulha, Fernanda B, Eizirik, Eduardo, Utz, Laura R. P
• Format: Article
• Language: English
• Subjects: 18S rDNA; Basal Bodies - ultrastructure; Brazil; Ciliophora; Ciliophora - classification; Ciliophora - genetics; Ciliophora - isolation & purification; Ciliophora - ultrastructure; Epistylis; Fresh Water - parasitology; freshwater; infraciliature; internal transcribed spacers; ITS; morphology; morphometry; Peritrichia; peritrichs; Phylogeny; ribosomal DNA; RNA, Ribosomal, 18S - genetics; Scopula; Sequence Analysis, DNA; surface water; vacuoles; zooids
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/jeu.12252

The peritrich ciliate Epistylis portoalegrensis n. sp. was found in two bodies of freshwater located in Porto Alegre, Southern Brazil. Morphological features were investigated using live and protargol‐stained specimens. The zooids presented a vase to cylindrical shape narrowed at the scopula, and a mean size of 131 × 37 μm in vivo. A C‐shaped macronucleus lay in the middle of the cell close to a single contractile vacuole. The oral infraciliature was typical for the genus, with all infundibular polykineties composed by three distinct rows of kinetosomes. Colonies are often nonbranched with no lateral stalk, carrying several zooids stemming from a single point. Specimens from the two sampling sites showed identical arrangement of the infraciliature, similar morphometry, identical 18S rDNA sequences, and a single nucleotide difference across the more variable ITS regions. Molecular phylogenetic analyses placed E. portoalegrensis in a well‐supported clade containing other Epistylis species, within the order Vorticellida.