Intracellular protease activity during the growth of Streptomyces coelicolor

Multiple intracellular proteases were produced by Streptomyces coelicolor throughout growth as surface cultures. Zymography revealed two constitutive, gelatinolytic proteases of approximate molecular masses 32.5 and 36.5 kDa. In addition, transient expression of a large (183.5 kDa) protease preceded...

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Bibliographic Details
Published in:Biotechnology letters 2001-02, Vol.23 (3), p.185-187
Main Authors: HOSKISSON, Paul A, MAKIN, Victoria, TAYLOR, Helen, SHARPLES, George P, HOBBS, Glyn
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
gelatinase
Metabolism. Enzymes
Microbiology
Miscellaneous
Mission oriented research
Proteases
Streptomyces coelicolor
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Summary:Multiple intracellular proteases were produced by Streptomyces coelicolor throughout growth as surface cultures. Zymography revealed two constitutive, gelatinolytic proteases of approximate molecular masses 32.5 and 36.5 kDa. In addition, transient expression of a large (183.5 kDa) protease preceded aerial mycelium formation and following this, during sporulation, an additional protease of mass 27.5 kDa was produced.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005603201902