Determination of Tryptophan in Raw Materials, Rat Brain and Human Plasma by RP-HPLC Technique

This paper describes tryptophan (TRP) estimation in raw human plasma and rat brain by reversed-phase high-performance liquid chromatography (RP-HPLC). Estimation was carried out on a Purospher STAR C18 column using water-acetonitrile (90:10 v/v, at pH 2.7) mixture at a rate of 1.5 mL/min as mobile p...

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Bibliographic Details
Published in:Journal of chromatographic science 2012-07, Vol.50 (6), p.531-537
Main Authors: Sultana, Najma, Arayne, M. Saeed, Khan, Moona Mehboob, Saleem, Darakhshan M., Mirza, Agha Zeeshan
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain
Brain Chemistry
Chromatography
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - methods
Eluents
Female
Human
Humans
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Sprague-Dawley
Raw materials
Tobacco, tobacco smoking
Toxicology
Tryptophan
Tryptophan - analysis
Tryptophan - blood
Ultraviolet detectors
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Summary:This paper describes tryptophan (TRP) estimation in raw human plasma and rat brain by reversed-phase high-performance liquid chromatography (RP-HPLC). Estimation was carried out on a Purospher STAR C18 column using water-acetonitrile (90:10 v/v, at pH 2.7) mixture at a rate of 1.5 mL/min as mobile phase. Eluents were monitored at 273 nm by an ultraviolet detector. The method was linear (R2 > 0.999), precise (intra-day and inter-day precision
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bms045