Study on the Adsorption Property of Lysozyme on Weak Cation Exchanger Based on Monodisperse Poly(glycidymethacrylate-co-ethylenedimethacrylate) Beads

A type of weak cation exchanger was prepared based on poly(glycidylmethacrylate-co-ethylenedimethacrylate). The effects of pH and ionic strength on the adsorption behavior were studied, and the results suggested that the adsorption of lysozyme onto a weak cation exchanger is electrostatic interactio...

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Bibliographic Details
Published in:Journal of chromatographic science 2013-02, Vol.51 (2), p.122-127
Main Authors: Zhang, Jing, Han, Yan-Ting, Bu, Xin-Li, Yue, Xuan-Feng
Format: Article
Language:English
Subjects:
Adsorption
Animals
Beads
Cation exchanging
Cations - chemistry
Chickens
Chromatography
Chromatography, Ion Exchange - instrumentation
Chromatography, Ion Exchange - methods
Exchange
Hydrogen-Ion Concentration
Ion exchangers
Isoelectric Point
Lysozyme
Methacrylates - chemistry
Microspheres
Muramidase - analysis
Muramidase - chemistry
Muramidase - pharmacokinetics
Osmolar Concentration
Polystyrenes - chemistry
Regression Analysis
Strength
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Summary:A type of weak cation exchanger was prepared based on poly(glycidylmethacrylate-co-ethylenedimethacrylate). The effects of pH and ionic strength on the adsorption behavior were studied, and the results suggested that the adsorption of lysozyme onto a weak cation exchanger is electrostatic interaction, and that the adsorption behavior is in accordance with the Langmuir adsorption model with a correlation coefficient greater than 0.99. It was also found that increasing ionic strength led to a decrease of the adsorption of lysozyme from 49.50 to 28.09 mg/g. Preliminary chromatographic experiments were conducted to test the separation properties of the weak cation exchanger, and the results demonstrated that the retention time of different proteins could be predicted in order of their isoelectric point.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bms115