Patterned Paper Sensors Printed with Long-Chain DNA Aptamers

There is growing interest in developing printable paper sensors to enable rapid testing of analytes for environmental, food safety, and clinical applications. A major challenge is to find suitable bioinks that are amenable to high‐speed printing and remain functional after printing. We report on a s...

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Bibliographic Details
Published in:Chemistry : a European journal 2015-05, Vol.21 (20), p.7369-7373
Main Authors: Carrasquilla, Carmen, Little, Jessamyn R. L., Li, Yingfu, Brennan, John D.
Format: Article
Language:English
Subjects:
aptamers
Aptamers, Nucleotide - chemistry
Biosensing Techniques
Biosensors
Chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
Fluorescence
Ink jet printers
Inkjet printing
Molecular structure
Multiplexing
Nucleic Acid Amplification Techniques - methods
paper sensors
Printing
Printing - methods
rolling circle amplification
Sensors
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Summary:There is growing interest in developing printable paper sensors to enable rapid testing of analytes for environmental, food safety, and clinical applications. A major challenge is to find suitable bioinks that are amenable to high‐speed printing and remain functional after printing. We report on a simple and effective approach wherein an aqueous ink composed of megadalton‐sized tandem repeating structure‐switching DNA aptamers (concatemeric aptamers) is used to rapidly create patterned paper sensors on filter paper by inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and fluorescence signaling to provide both qualitative and quantitative detection of small molecules and protein targets. The convenience of inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on‐demand printing of sensors even in remote locations. Spot the DNA (on paper): A “bioink” made of tandem repeating DNA aptamer reporters can easily be inkjet‐printed on paper in a pattern of choice and remain active while immobilized to function as a paper‐based biosensor (see figure).
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201500949