Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker

•An organic distributed feedback laser is used to detect ErbB2 protein biomarker.•An immunoassay for the specific detection of ErbB2 protein biomarker has been carried out.•A concentration limit of detection of 14ng/mL ErbB2 has been achieved. The human epidermal growth factor receptor 2 (ErbB2) pro...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2016-02, Vol.223, p.261-265
Main Authors: Retolaza, Aritz, Martinez-Perdiguero, Josu, Merino, Santos, Morales-Vidal, Marta, Boj, Pedro G., Quintana, José A., Villalvilla, José M., Díaz-García, María A.
Format: Article
Language:English
Subjects:
Biomarkers
Breast
Cancer
Distributed feedback
ErbB2 biomarker
Human
Label-free biosensors
Lasers
Lithography
Organic lasers
Perylenediimides
Polystyrene resins
Proteins
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Summary:•An organic distributed feedback laser is used to detect ErbB2 protein biomarker.•An immunoassay for the specific detection of ErbB2 protein biomarker has been carried out.•A concentration limit of detection of 14ng/mL ErbB2 has been achieved. The human epidermal growth factor receptor 2 (ErbB2) protein plays an important role in human malignancies. Its overexpression has been recognized as a feature of a malignant cancerous phenotype in breast cancer cell lines, and has become one of the most widely investigated clinical indicators of breast, ovarian, gastrointestinal and lung cancers. In this work a vertically emitting organic distributed feedback (DFB) laser has been used to detect the ErbB2 protein. This DFB laser consists of a polystyrene (PS) film containing a perylenediimide laser dye, deposited over a second-order one dimensional grating fabricated on fused silica by thermal-nanoimprint lithography and subsequent reactive ion etching processes. Specificity of the system to ErbB2 protein biomarker, achieved by functionalizing the PS with anti-ErbB2 monoclonal antibodies, is demonstrated. A concentration limit of detection for ErbB2 protein of 14ng/mL has been obtained, and no cross-reactivity has been observed with bovine serum albumin (BSA) and tumor necrosis factor alpha (TNFα) proteins. These findings open the possibility of using this type of biosensors in clinical applications.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2015.09.093