Analysis of the efficiency of photodynamic therapy using a microsystem for mono-, co- and mixed cultures

•We present the newly developed microsystem for evaluation of the efficiency of PDT procedures on three kinds of cell cultures in a single step.•The proposed microsystem allows to mimic in vivo conditions, where carcinoma cells grow next to non-malignant cell.•We described how different distances be...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2015-12, Vol.221, p.1356-1365
Main Authors: Jastrzebska, Elzbieta, Bulka, Magdalena, Rybicka, Natalia, Zukowski, Kamil
Format: Article
Language:English
Subjects:
Actuators
Biotechnology
Coculture
Culture
In vitro testing
Monoculture
Non-malignant-carcinoma models
Photodynamic therapy
Photodynamic therapy (PDT)
Precursors
Reactive oxygen species
Sensors
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Summary:•We present the newly developed microsystem for evaluation of the efficiency of PDT procedures on three kinds of cell cultures in a single step.•The proposed microsystem allows to mimic in vivo conditions, where carcinoma cells grow next to non-malignant cell.•We described how different distances between carcinoma and non-malignant cells influence on the efficiency of PDT procedures.•It was proven that the effectiveness of PDT procedures is dependent on the kind of the tested cells. Analysis of phototoxicity in monoculture can be performed both in a macroscale and a microsystem. To improve this kind of research, we propose fully integrated microsystem for evaluation of the efficiency of PDT procedures on three kinds of cell cultures (monoculture, coculture and mixed culture). Thanks to this the influence of PDT procedures on non-malignant cells, cultured in different distances with carcinoma cells was examined. 5-aminolevulinic acid (ALA) as a precursor of photosensitizer was used. In vitro non-malignant—carcinoma models with both A549-Balb/c 3T3 and A549-MRC5 cells were investigated in the designed microsystem. An influence of the carcinoma (A549) cells presence on the non-malignant cell viability was noticed for: A549-MRC5 coculture and A549-Balb/c 3T3 and A549-MRC5 mixed cultures. Our results confirm that the microsystem is useful for PDT procedures analysis not only in monoculture (like in standard laboratories) but also in coculture and mixed culture. The proposed microsystem is a new discovery, allowing us to establish, how different distances between two cell types influence on the efficiency of PDT procedure.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2015.08.022