Capillary Electrophoretic Determination of Antimigraine Formulations Containing Caffeine, Ergotamine, Paracetamol and Domperidone or Metoclopramide

• Published in: Journal of chromatographic science 2013-07, Vol.51 (6), p.502-510
• Main Authors: Sultan, Maha A., Maher, Hadir M., Alzoman, Nora Z., Alshehri, Mona M., Rizk, Mohamed S., Elshahed, Mona S., Olah, Ileana V.
• Format: Article
• Language: English
• Subjects: Acetaminophen - analysis; Acetaminophen - chemistry; Arrays; Caffeine; Caffeine - analysis; Caffeine - chemistry; Capillarity; Domperidone - analysis; Domperidone - chemistry; Drug Combinations; Drug Stability; Electrophoresis; Electrophoresis, Capillary - methods; Ergotamine; Ergotamine - analysis; Ergotamine - chemistry; Formulations; Hydrogen-Ion Concentration; Limit of Detection; Linear Models; Metoclopramide - analysis; Metoclopramide - chemistry; Migraine Disorders; Osmolar Concentration; Reproducibility of Results; Separation; Tablets; Tablets - chemistry; Tartrates - chemistry; Temperature
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/bms175

A novel, fast, sensitive and specific technique using capillary electrophoresis coupled to a diode array detector has been developed for the separation and simultaneous determination of two antimigraine mixtures in tablet formulation. The two combinations are ergotamine tartrate (ERG), caffeine (CAF) and paracetamol (PAR) with either domperidone (DOM), combination (I) or metoclopramide (MET), combination (II). The proposed method utilized a fused silica capillary (55 cm × 75 µm i.d.) and background electrolyte composed of phosphate buffer (25 mM, pH 9.8). The separation was achieved at 20 KV applied voltage and at 25°C. The described method was linear over the range of 1-80 and 2-100 µg/mL for CAF and MET, respectively, and 1-80 µg/mL for DOM, ERG and PAR. Intra-day and inter-day relative standard deviation (n = 5) was ≤1.10%. The limits of detection of CAF and PAR were 0.20 and 0.10 µg/mL, respectively, and 0.50 µg/mL for MET, DOM and ERG. Other aspects of analytical validation were also evaluated. The proposed method was successfully applied to the analysis of the two combinations in their tablets. Therefore, the proposed method is suitable for the routine control of these ingredients in multicomponent dosage forms.