Achievement of Superresolution in Optical-Thickness Measurement and Statistical Localization of Fluorophores in Holographic and Fluorescent Microscopy

We present the concept of combining the holographic and localization fluorescent microscopy. The ways to optimize the optical schemes and image recording regimes in the process of combining such interferometric and statistical methods are developed, which makes it possible to study the dynamics of t...

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Bibliographic Details
Published in:Radiophysics and quantum electronics 2015-01, Vol.57 (8-9), p.551-558
Main Authors: Dudenkova, V. V., Murav’eva, M. S., Rybnikov, A. I., Zakharov, Yu. N.
Format: Article
Language:English
Subjects:
Analysis
Astronomy
Astrophysics and Astroparticles
Chemical compounds
Dynamic structural analysis
Fluorescence
Hadrons
Heavy Ions
Lasers
Localization
Mathematical and Computational Physics
Measurement
Microscope and microscopy
Microscopy
Nuclear Physics
Observations and Techniques
Optical Devices
Optical properties
Optics
Photonics
Physics
Physics and Astronomy
Position (location)
Quantum electronics
Quantum Optics
Recording
Theoretical
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Description
Summary:We present the concept of combining the holographic and localization fluorescent microscopy. The ways to optimize the optical schemes and image recording regimes in the process of combining such interferometric and statistical methods are developed, which makes it possible to study the dynamics of the internal cell structure with transverse and longitudinal superresolution.
ISSN:0033-8443
1573-9120
DOI:10.1007/s11141-015-9538-9