NMR Signal Enhancement by Effective SABRE Labeling of Oligopeptides

Signal amplification by reversible exchange (SABRE) can enhance nuclear magnetic resonance signals by several orders of magnitude. However, until now this was limited to a small number of model target molecules. Here, a new convenient method for SABRE activation applicable to a variety of synthetic...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry : a European journal 2015-09, Vol.21 (36), p.12616-12619
Main Authors: Ratajczyk, Tomasz, Gutmann, Torsten, Bernatowicz, Piotr, Buntkowsky, Gerd, Frydel, Jaroslaw, Fedorczyk, Bartlomiej
Format: Article
Language:English
Subjects:
Activation
Amplification
Biocompatibility
Chemistry
Exchange
hyperpolarization
Magnetic Resonance Spectroscopy - methods
Marking
Models, Molecular
NMR
NMR spectroscopy
Nuclear magnetic resonance
Oligopeptides - chemistry
peptides
Pyridines - chemistry
signal amplification by reversible exchange
signal enhancement
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Signal amplification by reversible exchange (SABRE) can enhance nuclear magnetic resonance signals by several orders of magnitude. However, until now this was limited to a small number of model target molecules. Here, a new convenient method for SABRE activation applicable to a variety of synthetic model oligopeptides is demonstrated. For the first time, a highly SABRE‐active pyridine‐based biocompatible molecular framework is incorporated into synthetic oligopeptides. The SABRE activity is preserved, demonstrating the importance of such earmarking. Finally, a crucial exchange process responsible for SABRE activity is identified and discussed. How to enhance: Signal amplification by reversible exchange (SABRE) can enhance nuclear magnetic resonance signals by a few orders of magnitude. Here, an efficient protocol for the SABRE activation of SABRE non‐hyperpolarizable molecules is demonstrated. In this approach, SABRE‐active nicotinic‐ or, alternatively, isonicotinic acid was connected to the SABRE‐inactive oligopeptides. All SABRE‐labeled systems revealed hyperpolarization.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201501552