Immobilization of Ochrobactrum tritici As5 on PTFE thin films for arsenite biofiltration

Ochrobactrum tritici SCII24T bacteria is an environmental strain with high capacity to resist to arsenic (As) toxicity, which makes it able to grow in the presence of As(III). The inactivation of the two functional arsenite efflux pumps, ArsB and ACR3_1, resulted in the mutant O. tritici As5 exhibit...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2016-03, Vol.146, p.330-337
Main Authors: Branco, Rita, Sousa, Tânia, Piedade, Ana P., Morais, Paula V.
Format: Article
Language:English
Subjects:
Arsenic
Arsenic bioaccumulation
Arsenic resistance
Arsenites - metabolism
As(III) efflux pumps
Bacteria
Bacteria immobilization
Biofilms
Biotool
Deposition
Immobilization
Ochrobactrum - metabolism
Polymers
Polytetrafluoroethylene - metabolism
Polytetrafluoroethylenes
PTFE
Thin films
Toxicity
Waste Disposal, Fluid - methods
Water Pollutants, Chemical - metabolism
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Summary:Ochrobactrum tritici SCII24T bacteria is an environmental strain with high capacity to resist to arsenic (As) toxicity, which makes it able to grow in the presence of As(III). The inactivation of the two functional arsenite efflux pumps, ArsB and ACR3_1, resulted in the mutant O. tritici As5 exhibiting a high accumulation of arsenite. This work describes a method for the immobilization of the mutant cells O. tritici As5, on a commercial polymeric net after sputtered modified by the deposition of poly(tetrafluoroethylene) (PTFE) thin films, and demonstrates the capacity of immobilized cells to accumulate arsenic from solutions. Six different set of deposition parameters for PTFE thin films were developed and tested in vitro regarding their ability to immobilize the bacterial cells. The surface that exhibited a mild zeta potential value, hydrophobic characteristics, the lowest surface free energy but with a high polar component and the appropriate ratio of chemical reactive groups allowed cells to proliferate and to grow as a biofilm. These immobilized cells maintained their ability to accumulate the surrounding arsenite, making it a great arsenic biofilter to be used in bioremediation processes. •Ochrobactrum tritici As5 with inactivated arsenite efflux pumps hiperaccumulates arsenic.•Sputtering modification of polyethylene net with PTFE.•New method of immobilization of metabolic active bacterial cells.•Immobilized cells maintained their ability to accumulate the surrounding arsenite.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2015.12.025