Highly sensitive detection of epidermal growth factor receptor expression levels using a capacitance sensor

We developed a capacitance sensor with parallel plate geometry to measure epithermal growth factor receptor (EGFR) expression levels on cell membrane in real-time. We first proved correlations between capacitance changes and cell numbers settled down between electrodes, and then observed capacitance...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2015-03, Vol.209, p.438-443
Main Authors: Shin, Dong-Myeong, Shin, Yong-Cheol, Lee, Jong-Ho, Kim, Tae-Hyun, Han, Dong-Wook, Kim, Jong-Man, Kim, Hyung Kook, Kim, Kyujung, Hwang, Yoon-Hwae
Format: Article
Language:English
Subjects:
Actuators
Capacitance
Capacitance biosensor
Electrodes
Epithermal growth factor
Fibroblastic cells
Growth factors
Membranes
Osteoblastic cells
Real time
Receptor expression level
Receptors
Sensors
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Summary:We developed a capacitance sensor with parallel plate geometry to measure epithermal growth factor receptor (EGFR) expression levels on cell membrane in real-time. We first proved correlations between capacitance changes and cell numbers settled down between electrodes, and then observed capacitance changes elicited by interactions between EGFR on membrane and EGF proteins in real time. Consequently, we confirmed that the EGFR expression levels of varied typed cells were successfully quantified. This approach can effectively distinguish differences of EGFR levels of cancer cells and normal cells in real-time. Also, up to 600% sensitivity enhancements and around 2.2h on average sensing time saving were achieved by using the capacitance sensor over a conventional immunoassay technique. Such a capacitance biosensor can be extended to broad fields where the receptor–antibody reactions, the receptor–virus reactions or DNA hybridizations are involved.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2014.12.001