Simultaneous amperometric determination of malic and gluconic acids in wine using screen-printed carbon electrodes

The selective and simultaneous amperometric determination of malic and gluconic acids was carried out using a screen-printed design with two working carbon electrodes. One of the two working electrodes was modified with gold nanoparticles, tetrathiafulvalene (TTF) and malate quinone oxido-reductase...

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Published in:Sensors and actuators. B, Chemical Chemical, 2015-05, Vol.211, p.250-254
Main Authors: Molinero-Abad, Begoña, Alonso-Lomillo, M. Asunción, Domínguez-Renedo, Olga, Arcos-Martínez, M. Julia
Format: Article
Language:English
Subjects:
Arrays
Carbon
Electrical measurements
Electrodes
Enzymes
Gluconate dehydrogenase
Gluconic acid
Malate quinone oxido-reductase
Malic acid
Screen-printed carbon electrodes
Wine
Wines
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cited_by cdi_FETCH-LOGICAL-c396t-acd2e11db3939f68d4a6de856d8b42c8bb3dd3c837597738294321126d92c9b33
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container_title Sensors and actuators. B, Chemical
container_volume 211
creator Molinero-Abad, Begoña
Alonso-Lomillo, M. Asunción
Domínguez-Renedo, Olga
Arcos-Martínez, M. Julia
description The selective and simultaneous amperometric determination of malic and gluconic acids was carried out using a screen-printed design with two working carbon electrodes. One of the two working electrodes was modified with gold nanoparticles, tetrathiafulvalene (TTF) and malate quinone oxido-reductase enzyme for the sensitive detection of malic acid. Analogously, the other working electrode was modified with TTF and gluconate dehydrogenase enzyme for the sensitive detection of gluconic acid. The array of biosensors responds to gluconic and malic acids giving each anodic currents (+100mV and pH 6.5), with detection capabilities of 1.89μM (α=β=0.05) and 0.79μM to malic and gluconic acids, respectively, at room temperature. The method has been applied to the determination of both analytes in wine, obtaining successful results.
doi_str_mv 10.1016/j.snb.2015.01.101
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1873-3077
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source Elsevier
subjects Arrays
Carbon
Electrical measurements
Electrodes
Enzymes
Gluconate dehydrogenase
Gluconic acid
Malate quinone oxido-reductase
Malic acid
Screen-printed carbon electrodes
Wine
Wines
title Simultaneous amperometric determination of malic and gluconic acids in wine using screen-printed carbon electrodes
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