Highly sensitive microcantilever biosensors with enhanced sensitivity for detection of human papilloma virus infection

This paper demonstrates a piezoresistive microcantilever biosensor for a potential of the early detection of HPV infection. Gold was patterned on the top surface of the microcantilever to define the sensing area. Then, a self-assembled monolayer (SAM) was coated on the gold surface for the attachmen...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2015-12, Vol.221, p.1372-1383
Main Authors: Kim, Hyung Hoon, Jeon, Hyeong Jin, Cho, Hyun Kook, Cheong, Jea Hun, Moon, Hyeong Soon, Go, Jeung Sang
Format: Article
Language:English
Subjects:
Beads
Biosensors
Deoxyribonucleic acid
Detection
Fragments
Gold
Human papilloma virus
Human papillomavirus
Magnetic force
Microcantilever
Sensors
Signal amplification
Surface chemistry
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Summary:This paper demonstrates a piezoresistive microcantilever biosensor for a potential of the early detection of HPV infection. Gold was patterned on the top surface of the microcantilever to define the sensing area. Then, a self-assembled monolayer (SAM) was coated on the gold surface for the attachment of a gold binding protein (GBP) which was functionally activated with carboxyl groups and amine groups to bind probe molecules such as antibodies, oligonucleotide probes, DNA, and proteins. The surface-activated magnetic beads were introduced to the sensing area with DNA fragments of the HPV-infected cells. The magnetic force was induced to the surface-activated magnetic beads bound with probe molecules and HPV DNA fragments by placing a permanent magnet. The electrical signal output measured from the piezoresistive sensor integrated in the microcantilever was amplified 1000 times compared with non-HPV solution with a high SN ratio.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2015.08.014