HPLC Method for Naproxen Determination in Human Plasma and Its Application to a Pharmacokinetic Study in Turkey

A simple high-performance liquid chromatography method has been developed for the determination of naproxen in human plasma. The method was validated on an Ace C18 column using ultraviolet detection. The mobile phase consisted of 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid–ace...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatographic science 2014-08, Vol.52 (7), p.584-589
Main Authors: Yilmaz, Bilal, Asci, Ali, Erdem, Ali Fuat
Format: Article
Language:English
Subjects:
Adult
Buffers
Calibration
Chromatography
Chromatography, High Pressure Liquid - methods
Extraction
Human
Humans
Limit of Detection
Linear Models
Liquid chromatography
Male
Naproxen
Naproxen - blood
Naproxen - chemistry
Naproxen - pharmacokinetics
Phosphates
Reproducibility of Results
Turkey
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple high-performance liquid chromatography method has been developed for the determination of naproxen in human plasma. The method was validated on an Ace C18 column using ultraviolet detection. The mobile phase consisted of 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid–acetonitrile (65:35, v/v). The calibration curve was linear between the concentration ranges of 0.10 and 5.0 µg/mL. Intra-day and inter-day precision values for naproxen in plasma were less than 4.84, and accuracy (relative error) was better than 3.67%. The extraction recovery values of naproxen from human plasma were between 91.0 and 98.9%. The limits of detection and quantification of naproxen were 0.03 and 0.10 µg/mL, respectively. Also, this assay was applied to determine the pharmacokinetic parameters of naproxen in six healthy Turkish volunteers who had been given 220 mg of naproxen.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmt080