Nanostructured multilayer polyelectrolyte films with silver nanoparticles as antibacterial coatings

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2016-01, Vol.137, p.158-166
• Main Authors: Kruk, Tomasz, Szczepanowicz, Krzysztof, Kręgiel, Dorota, Szyk-Warszyńska, L., Warszyński, Piotr
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Anti-Bacterial Agents - chemistry; Antiinfectives and antibacterials; Antimicrobial coatings; Bacteria; Coatings; Electrolytes - chemistry; Ellipsometry; Escherichia coli; Layer-by-layer technique; Metal Nanoparticles; Microbalances; Multilayers; Nanoparticles; Nanostructures; Polyelectrolyte multilayers; Polyelectrolytes; QCM-D; Silver; Silver - chemistry; Silver nanoparticles adsorption
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.06.016

•Polyelectrolyte film with silver nanoparticles as antibacterial coatings is presented.•Nanocomposite films prevent of microbial colonization on treated surfaces.•LbL is a versatile technique for formation of biologically active surface nanostructures.•Future challenges of nanocomposite coatings for antibacterial applications are proposed. Ultrathin polyelectrolyte films containing silver nanoparticles appear to be a promising material for antimicrobial coatings used in the medical area. The present work is focused on the formation of multilayer polyelectrolyte films using: polyethyleneimine (PEI) as polycation, Poly(sodium 4-styrenesulfonate) (PSS) as polyanions and negatively charged silver nanoparticles (AgNPs), which led to the polyelectrolyte-silver nanocomposite coatings. The film thickness and mass were measured by ellipsometry and quartz crystal microbalance with dissipation monitoring (QCM-D) and the structure and morphology of films were visualized using scanning electron microscopy (SEM). Systematic increase of the UV–Vis absorption confirmed formation of the consecutive layers of the film. The analysis of bacteria cell adhesion to films surface was done by the luminometry measurement. Three gram-negative bacterial strains with strong adhesive properties were used in this study: Escherichia coli, Aeromonas hydrophila, and Asaia lannenesis. It was found that nanocomposite films have antimicrobial properties, which makes them very interesting for a number of practical applications, e.g. for the prevention of microbial colonization on treated surfaces.