MicroRNA-143 promotes apoptosis of osteosarcoma cells by caspase-3 activation via targeting Bcl-2

Abstract Osteosarcoma is the most common malignant bone tumor. In recent years, although a lot of research in the mechanism of osteosarcoma development and metastasis had been done, the molecular mechanisms are still elusive. MicroRNAs (miRs), as small noncoding RNA sequences, are dysregulated in va...

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Published in:Biomedicine & pharmacotherapy 2016-05, Vol.80, p.8-15
Main Authors: Li, Wei-hua, Wu, Hao-jie, Li, Yu-xia, Pan, Hua-gang, Meng, Tao, Wang, Xiao
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
Base Sequence
Bcl-2
Caspase 3 - metabolism
Cell Line, Tumor
Cell Proliferation
Enzyme Activation
Gene Expression Regulation, Neoplastic
Humans
Internal Medicine
Medical Education
Mice, Inbred BALB C
Mice, Nude
MicroRNAs - genetics
MicroRNAs - metabolism
MiR
Neoplasm Metastasis
Osteosarcoma
Osteosarcoma - enzymology
Osteosarcoma - genetics
Osteosarcoma - pathology
Proto-Oncogene Proteins c-bcl-2 - metabolism
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Summary:Abstract Osteosarcoma is the most common malignant bone tumor. In recent years, although a lot of research in the mechanism of osteosarcoma development and metastasis had been done, the molecular mechanisms are still elusive. MicroRNAs (miRs), as small noncoding RNA sequences, are dysregulated in various diseases, including cancer, negatively modulating the target genes expression by posttranscriptional repression. MicroRNA-143 (miR-143) has been reported to be reduced in cancers, including pituitary, colorectal, prostate cancer and cervical. We were aimed to detect the effects of miR-143 on osteosarcoma cell invasion and migration as well as to indicate the potential molecular mechanisms by which miR-143 regulated osteosarcoma. After miR-143 transfection, the cancer cells migration and invasion were examined. And Western blot, RT-PCR, flow cytometry and immunochemistry assays were performed to analyze the role of miR-143 in osteosarcoma progression. The results suggested that miR-143 expressed lessly in osteosarcoma cell lines and could suppress cell migration and invasion in U2-OS and MG-63 cells. To our knowledge, it was the first time to target Bcl-2 directly to explore the underlying mechanism by which miR-143 performed its role to induce apoptosis in tumor cells, thus improving osteosarcoma progression. The present study indicated that miR-143 could inhibit Bcl-2 expression, causing Caspas3 activation, thus inducing apoptosis in osteosarcoma cells. MiR-143 may therefore sreve as a potential biomarker for osteosarcoma, and the regulation of its expression might be a novel therapeutic strategy for osteosarcoma treatment.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2016.03.001