Uterine lavage is efficient to recover endometrial cytology sample and does not interfere with fertility rate after artificial insemination in cows

• Published in: Theriogenology 2016-06, Vol.85 (9), p.1549-1554
• Main Authors: Thomé, Helder Esteves, de Arruda, Rubens Paes, de Oliveira, Bruna Marcele Martins, Maturana Filho, Milton, de Oliveira, Guilherme Cain, Guimarães, Carina de Fátima, de Carvalho Balieiro, Júlio César, Azedo, Milton Ricardo, Pogliani, Fábio Celidônio, Celeghini, Eneiva Carla Carvalho
• Format: Article
• Language: English
• Subjects: Animals; Cattle; Cow; Cytobrush; Doppler ultrasound; Endometritis; Endometritis - pathology; Endometrium - diagnostic imaging; Endometrium - pathology; Female; Fertility; Insemination, Artificial - veterinary; Pregnancy; Pregnancy Rate; Regional Blood Flow; Therapeutic Irrigation; Uterine hemodynamic; Uterus - blood supply; Uterus - diagnostic imaging; Uterus - pathology
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2016.01.014

Productivity rates directly depend on the fertility of a herd, which in turn can be influenced by many factors. Semen deposited in the female reproductive tract is foreign to the body and, in response to this invasion, produces an inflammatory reaction, which is characterized by rapid infusion of polymorphonuclear (PMN) cells. Techniques to obtain an endometrial sample are usually invasive and can mask the true inflammatory response. Ultrasound is a noninvasive technique and can contribute to the diagnosis of postartificial insemination (AI) inflammatory response in cattle. The present study was divided into two experiments. The aim of experiment 1 was to compare two methods of endometrial cytology collection, uterine cytobrush (UC) and uterine lavage (UL), and their effects on uterine hemodynamics that provide information about blood flow. The two methods were evaluated by Doppler ultrasound using the spectral and color modes. For that purpose, 19 Nellore cows were synchronized for timed AI and subjected to UC (n = 9) or UL (n = 10). The techniques were performed 4 hours after AI. The results showed that both techniques allow collection of a good quality sample and with enough PMN cells to perform counting. More PMN cells were obtained by UL than UC. There was no difference in uterine blood flow between the UC and UL groups in any of the periods evaluated (34 hours before and 4, 24, and 48 hours after collection of uterine sample). On the basis of results of experiment 1, the effect of UL on fertility was studied in experiment 2. A total of 128 Nellore cows were synchronized for TAI; 35 cows were subjected to endometrial cytology by UL 4 hours after AI, and 93 were not submitted to any procedure (control). Pregnancy diagnosis was performed by transrectal ultrasound 30 days after AI. Pregnancy rates did not differ between UL (54.29%) and control (56.99%) groups. The results of this study showed that UL allows the collection of more representative cells of the surface of the uterus than UC technique and causes no damage to the reproductive tract. Moreover, UL did not affect pregnancy rate when performed 4 hours after AI.