Production of Drosophila melanogaster acetylcholinesterase in Saccharomyces cerevisiae

Biologically active acetylcholinesterase from Drosophila melanogaster was constitutively expressed from a high copy number plasmid in Saccharomyces cerevisiae. Cellular metabolism and plasmid copy number were unaffected by expression. Based on comparative PMSF inhibition, the K^sub cat^ value (6430...

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Bibliographic Details
Published in:Biotechnology letters 2001-03, Vol.23 (6), p.417-422
Main Authors: STOPPS, K. C. A, CURRAN, B. P. G, KHALAWAN, S. A, MALCOLM, C. A
Format: Article
Language:English
Subjects:
acetylcholinesterase
Biological and medical sciences
Biotechnology
Drosophila melanogaster
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Methods. Procedures. Technologies
Production of selected enzymes
Saccharomyces cerevisiae
Torpedo
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Summary:Biologically active acetylcholinesterase from Drosophila melanogaster was constitutively expressed from a high copy number plasmid in Saccharomyces cerevisiae. Cellular metabolism and plasmid copy number were unaffected by expression. Based on comparative PMSF inhibition, the K^sub cat^ value (6430 mol s^sup -1^) was commensurate with human and Torpedo acetylcholinesterases. A 1.7 knt truncated mRNA transcript was observed on Northern blot analysis.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010330015598