Development of an enzyme-linked glycosorbent method to monitor the inhibition of sialic acid-dependent Helicobacter pylori adhesion

Horseradish peroxidase conjugation with fetuin, which expresses sialic acid-dependent binding specificity to Helicobacter pylori, was used to develop an enzyme-linked glycosorbent method. This method yielded results that were consistent with those from a hemagglutination assay using a microscope and...

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Bibliographic Details
Published in:Biotechnology letters 2001-04, Vol.23 (7), p.507-511
Main Authors: WOO, Jeung S, HA, Byung H, KIM, Tae G, LIM, Yoong H, KIM, Kyung H
Format: Article
Language:English
Subjects:
Adhesion
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Carbohydrates
fetuin
Fundamental and applied biological sciences. Psychology
Helicobacter pylori
Microbiology
Monitoring methods
Saccharides
sialic acid
sialic acids
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Summary:Horseradish peroxidase conjugation with fetuin, which expresses sialic acid-dependent binding specificity to Helicobacter pylori, was used to develop an enzyme-linked glycosorbent method. This method yielded results that were consistent with those from a hemagglutination assay using a microscope and allowed the quantitative analysis of inhibitors of sialic acid-dependent Helicobacter pylori adhesion to host cells. The results of inhibitor screening with carbohydrates, including commercially available polysaccharides and extracted from various sources, displayed not only the relative inhibition potencies among carbohydrates, but also their respective concentration-dependencies.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010360412969