Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.)

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cu...

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Bibliographic Details
Published in:Plant cell reports 2004-11, Vol.23 (5), p.291-296
Main Authors: Han, J.S, Oh, D.G, Mok, I.G, Park, H.G, Kim, C.K
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
adventitious shoots
Aminobutyrates - pharmacology
Benzyl Compounds
Biological and medical sciences
Biotechnology
Cotyledon - growth & development
cotyledons
Cucurbitaceae - genetics
Cucurbitaceae - growth & development
Cultivars
culture media
Culture Media - chemistry
Culture Media - pharmacology
Culture Techniques - methods
Eukaryotic cell cultures
explants
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Genotype
herbaceous plants
in vitro regeneration
indole acetic acid
Indoleacetic Acids - pharmacology
Kinetin
Lagenaria siceraria
Methods. Procedures. Technologies
micropropagation
Miscellaneous
Naphthaleneacetic Acids - pharmacology
naphthaleneactic acid
Plant cells and fungal cells
Plant Growth Regulators - pharmacology
plant regeneration
Plant Shoots - growth & development
Ploidies
Purines
rooting
Seedlings
Shoots
silver nitrate
Silver Nitrate - pharmacology
vines
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Summary:Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO(3) under a 16-h photoperiod. After 3-4 weeks of culture, 21.9-80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2-3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO(3) were diploid.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0846-3