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SOS Mutator DNA Polymerase IV Functions in Adaptive Mutation and Not Adaptive Amplification
Adaptive point mutation and amplification are induced responses to environmental stress, promoting genetic changes that can enhance survival. A specialized adaptive mutation mechanism has been documented in one Escherichia coli assay, but its enzymatic basis remained unclear. We report that the SOS-...
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Published in: | Molecular cell 2001-03, Vol.7 (3), p.571-579 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Adaptive point mutation and amplification are induced responses to environmental stress, promoting genetic changes that can enhance survival. A specialized adaptive mutation mechanism has been documented in one
Escherichia coli assay, but its enzymatic basis remained unclear. We report that the SOS-inducible, error-prone DNA polymerase (pol) IV, encoded by
dinB, is required for adaptive point mutation in the
E. coli lac operon. A nonpolar
dinB mutation reduces adaptive mutation frequencies by 85% but does not affect adaptive amplification, growth-dependent mutation, or survival after oxidative or UV damage. We show that pol IV, together with the major replicase, pol III, can account for all adaptive point mutations at
lac. The results identify a role for pol IV in inducible genetic change. |
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ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/S1097-2765(01)00204-0 |