Incidence of Mycobacterium paratuberculosis in raw sheep and goats’ milk in England, Wales and Northern Ireland

A blind survey of 104 raw sheep and goats’ milk samples (90 goat, 14 sheeps) from bulk tanks on farms throughout England, Wales and Northern Ireland was carried out over a 5-month period (January–May 1998) in order to determine the incidence of Mycobacterium paratuberculosis. Each milk sample (100 m...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2001-03, Vol.79 (2), p.123-131
Main Authors: Grant, I.R, O’Riordan, L.M, Ball, H.J, Rowe, M.T
Format: Article
Language:English
Subjects:
Animal bacterial diseases
Animals
Bacterial diseases
Biological and medical sciences
British Isles
Decontamination and culture
England
Female
Food Microbiology
Fundamental and applied biological sciences. Psychology
Goats
Human bacterial diseases
Immunomagnetic PCR
Immunomagnetic Separation - veterinary
Incidence
Infectious diseases
Medical sciences
Microbiology
Milk - microbiology
Mycobacterium avium subsp. paratuberculosis - isolation & purification
Mycobacterium paratuberculosis
Northern Ireland
Polymerase Chain Reaction - veterinary
Raw goats’ milk
Raw sheep’s milk
Sheep
Tuberculosis and atypical mycobacterial infections
Wales
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A blind survey of 104 raw sheep and goats’ milk samples (90 goat, 14 sheeps) from bulk tanks on farms throughout England, Wales and Northern Ireland was carried out over a 5-month period (January–May 1998) in order to determine the incidence of Mycobacterium paratuberculosis. Each milk sample (100 ml) was divided into two 50 ml portions. One portion was decontaminated with 0.75% hexadecylpyridinium chloride for 5 h before culture on slopes of Herrold’s egg yolk medium and in BACTEC radiometric medium. The second portion was subjected to immunomagnetic separation followed by IS900 PCR (IMS–PCR). The IMS–PCR assay was employed in order to provide a more rapid indication of the presence of M. paratuberculosis in each milk sample than is possible by culture. Information on the Johne’s disease status of the sheep and goat herds that took part in the survey was not sought at the time of milk sampling. However, it subsequently emerged that at least some of the herds whose bulk milk was tested during this study were previously or currently infected with Johne’s disease. Overall, during this survey one raw goats’ milk sample tested positive for the presence of M. paratuberculosis by IMS–PCR (
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(00)00344-8