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Multiplex PCR for Typing Strains of Toxoplasma gondii

A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-04, Vol.43 (4), p.1940-1943
Main Authors: Ajzenberg, Daniel, Dumètre, Aurélien, Dardé, Marie-Laure
Format: Article
Language:English
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Summary:A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.4.1940-1943.2005