Skin Barrier Defects Caused by Keratinocyte-Specific Deletion of ADAM17 or EGFR Are Based on Highly Similar Proteome and Degradome Alterations

• Published in: Journal of proteome research 2016-05, Vol.15 (5), p.1402-1417
• Main Authors: Tholen, Stefan, Wolf, Cristina, Mayer, Bettina, Knopf, Julia D, Löffek, Stefanie, Qian, Yawen, Kizhakkedathu, Jayachandran N, Biniossek, Martin L, Franzke, Claus-Werner, Schilling, Oliver
• Format: Article
• Language: English
• Subjects: ADAM17 Protein - deficiency; ADAM17 Protein - genetics; Animals; Epidermis - pathology; Gene Deletion; Keratinocytes; Mice; Proteolysis; Proteome - analysis; Proteome - metabolism; Receptor, Epidermal Growth Factor - deficiency; Receptor, Epidermal Growth Factor - genetics
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/acs.jproteome.5b00691

Keratinocyte-specific deletion of ADAM17 in mice impairs terminal differentiation of keratinocytes leading to severe epidermal barrier defects. Mice deficient for ADAM17 in keratinocytes phenocopy mice with a keratinocyte-specific deletion of epidermal growth factor receptor (EGFR), which highlights the role of ADAM17 as a “ligand sheddase” of EGFR ligands. In this study, we aim for the first proteomic/degradomic approach to characterize the disruption of the ADAM17–EGFR signaling axis and its consequences for epidermal barrier formation. Proteomic profiling of the epidermal proteome of mice deficient for either ADAM17 or EGFR in keratinocytes at postnatal days 3 and 10 revealed highly similar protein alterations for ADAM17 and EGFR deficiency. These include massive proteome alterations of structural and regulatory components important for barrier formation such as transglutaminases, involucrin, filaggrin, and filaggrin-2. Cleavage site analysis using terminal amine isotopic labeling of substrates revealed increased proteolytic processing of S100 fused-type proteins including filaggrin-2. Alterations in proteolytic processing are supported by altered abundance of numerous proteases upon keratinocyte-specific Adam17 or Egfr deletion, among them kallikreins, cathepsins, and their inhibitors. This study highlights the essential role of proteolytic processing for maintenance of a functional epidermal barrier. Furthermore, it suggests that most defects in formation of the postnatal epidermal barrier upon keratinocyte-specific ADAM17 deletion are mediated via EGFR.