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Anti‐dengue virus serotype 2 activity and mode of action of a novel peptide
Aim To identify a novel antiviral peptide against dengue virus serotype 2 (DENV‐2) by screening a phage display peptide library and to evaluate its in vitro antiviral activity and mode of action. Methods and Results A phage display peptide library was biopanned against purified DENV‐2 and resulted i...
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| Published in: | Journal of applied microbiology 2015-10, Vol.119 (4), p.1170-1180 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4911-cbb1b7556e2838c817be018fc6e259ea679caa7ecb6c6feb4eb46bd60a2663a43 |
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| cites | cdi_FETCH-LOGICAL-c4911-cbb1b7556e2838c817be018fc6e259ea679caa7ecb6c6feb4eb46bd60a2663a43 |
| container_end_page | 1180 |
| container_issue | 4 |
| container_start_page | 1170 |
| container_title | Journal of applied microbiology |
| container_volume | 119 |
| creator | Chew, M.‐F. Tham, H.‐W. Rajik, M. Sharifah, S.H. |
| description | Aim
To identify a novel antiviral peptide against dengue virus serotype 2 (DENV‐2) by screening a phage display peptide library and to evaluate its in vitro antiviral activity and mode of action.
Methods and Results
A phage display peptide library was biopanned against purified DENV‐2 and resulted in the identification and selection of a peptide (peptide gg‐ww) for further investigation. ELISA was performed, and peptide gg‐ww was shown to possess the highest binding affinity against DENV‐2. Thus, peptide gg‐ww was synthesized for cytotoxicity and antiviral assays. Virus plaque reduction assay, real‐time PCR and immunofluorescence assay were used to investigate the inhibitory effect of peptide gg‐ww on DENV‐2 infection in Vero cells. Three different assays (pre‐, simultaneous and post‐treatments assays) were performed to investigate the peptide's mode of action. Results indicated that peptide gg‐ww possessed strong antiviral activity with a ~96% inhibition rate, which was achieved at 250 μmol l−1. Viral replication was inhibited during a simultaneous treatment assay, indicating that the entry of the virus was impeded by this peptide.
Conclusions
Peptide gg‐ww displayed antiviral action against DENV‐2 by targeting an early stage of viral replication (i.e. during viral entry).
Significance and Impact of the Study
Peptide gg‐ww may represent a new therapeutic candidate for the treatment of DENV infections and is a potential candidate to be developed as a peptide drug. |
| doi_str_mv | 10.1111/jam.12921 |
| format | article |
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To identify a novel antiviral peptide against dengue virus serotype 2 (DENV‐2) by screening a phage display peptide library and to evaluate its in vitro antiviral activity and mode of action.
Methods and Results
A phage display peptide library was biopanned against purified DENV‐2 and resulted in the identification and selection of a peptide (peptide gg‐ww) for further investigation. ELISA was performed, and peptide gg‐ww was shown to possess the highest binding affinity against DENV‐2. Thus, peptide gg‐ww was synthesized for cytotoxicity and antiviral assays. Virus plaque reduction assay, real‐time PCR and immunofluorescence assay were used to investigate the inhibitory effect of peptide gg‐ww on DENV‐2 infection in Vero cells. Three different assays (pre‐, simultaneous and post‐treatments assays) were performed to investigate the peptide's mode of action. Results indicated that peptide gg‐ww possessed strong antiviral activity with a ~96% inhibition rate, which was achieved at 250 μmol l−1. Viral replication was inhibited during a simultaneous treatment assay, indicating that the entry of the virus was impeded by this peptide.
Conclusions
Peptide gg‐ww displayed antiviral action against DENV‐2 by targeting an early stage of viral replication (i.e. during viral entry).
Significance and Impact of the Study
Peptide gg‐ww may represent a new therapeutic candidate for the treatment of DENV infections and is a potential candidate to be developed as a peptide drug.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12921</identifier><identifier>PMID: 26248692</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; antiviral ; Antiviral Agents - chemistry ; Antiviral Agents - pharmacology ; Antiviral drugs ; Cercopithecus aethiops ; Dengue - virology ; Dengue fever ; Dengue virus ; Dengue Virus - classification ; Dengue Virus - drug effects ; Dengue Virus - isolation & purification ; Dengue Virus - physiology ; drug ; Humans ; Molecular Sequence Data ; peptide ; Peptides ; Peptides - chemistry ; Peptides - pharmacology ; phage ; Pharmacology ; Serogroup ; Vero Cells ; Viral Plaque Assay ; Virus Replication - drug effects</subject><ispartof>Journal of applied microbiology, 2015-10, Vol.119 (4), p.1170-1180</ispartof><rights>2015 The Society for Applied Microbiology</rights><rights>2015 The Society for Applied Microbiology.</rights><rights>Copyright © 2015 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4911-cbb1b7556e2838c817be018fc6e259ea679caa7ecb6c6feb4eb46bd60a2663a43</citedby><cites>FETCH-LOGICAL-c4911-cbb1b7556e2838c817be018fc6e259ea679caa7ecb6c6feb4eb46bd60a2663a43</cites><orcidid>0000-0002-8499-2152</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26248692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chew, M.‐F.</creatorcontrib><creatorcontrib>Tham, H.‐W.</creatorcontrib><creatorcontrib>Rajik, M.</creatorcontrib><creatorcontrib>Sharifah, S.H.</creatorcontrib><title>Anti‐dengue virus serotype 2 activity and mode of action of a novel peptide</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
To identify a novel antiviral peptide against dengue virus serotype 2 (DENV‐2) by screening a phage display peptide library and to evaluate its in vitro antiviral activity and mode of action.
Methods and Results
A phage display peptide library was biopanned against purified DENV‐2 and resulted in the identification and selection of a peptide (peptide gg‐ww) for further investigation. ELISA was performed, and peptide gg‐ww was shown to possess the highest binding affinity against DENV‐2. Thus, peptide gg‐ww was synthesized for cytotoxicity and antiviral assays. Virus plaque reduction assay, real‐time PCR and immunofluorescence assay were used to investigate the inhibitory effect of peptide gg‐ww on DENV‐2 infection in Vero cells. Three different assays (pre‐, simultaneous and post‐treatments assays) were performed to investigate the peptide's mode of action. Results indicated that peptide gg‐ww possessed strong antiviral activity with a ~96% inhibition rate, which was achieved at 250 μmol l−1. Viral replication was inhibited during a simultaneous treatment assay, indicating that the entry of the virus was impeded by this peptide.
Conclusions
Peptide gg‐ww displayed antiviral action against DENV‐2 by targeting an early stage of viral replication (i.e. during viral entry).
Significance and Impact of the Study
Peptide gg‐ww may represent a new therapeutic candidate for the treatment of DENV infections and is a potential candidate to be developed as a peptide drug.</description><subject>Animals</subject><subject>antiviral</subject><subject>Antiviral Agents - chemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral drugs</subject><subject>Cercopithecus aethiops</subject><subject>Dengue - virology</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Dengue Virus - classification</subject><subject>Dengue Virus - drug effects</subject><subject>Dengue Virus - isolation & purification</subject><subject>Dengue Virus - physiology</subject><subject>drug</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>peptide</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Peptides - pharmacology</subject><subject>phage</subject><subject>Pharmacology</subject><subject>Serogroup</subject><subject>Vero Cells</subject><subject>Viral Plaque Assay</subject><subject>Virus Replication - drug effects</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqN0c9LwzAUB_AgipvTg_-ABLzooS5J26Q9juFPNrzouaTpq3S0TU3aSW_-Cf6N_iVm3fQgCIZAHo8PX5I8hE4puaJuTVeyuqIsZnQPjanPQ49xwfaHOvBCItgIHVm7IoT6JOSHaMQ4CyIeszFazuq2-Hz_yKB-6QCvC9NZbMHotm8AMyxVW6yLtseyznClM8A6H5q6Hipc6zWUuIGmLTI4Rge5LC2c7M4Jer65fprfeYvH2_v5bOGpIKbUU2lKUxGGHFjkRyqiIgVCo1y5RhiD5CJWUgpQKVc8hzRwm6cZJ5Jx7svAn6CLbW5j9GsHtk2qwiooS1mD7mxCRSRiISgV_6A05JyQiDh6_ouudGdq95CN8t3NIz926nKrlNHWGsiTxhSVNH1CSbIZR-LGkQzjcPZsl9ilFWQ_8vv_HZhuwVtRQv93UvIwW24jvwAzNpNW</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Chew, M.‐F.</creator><creator>Tham, H.‐W.</creator><creator>Rajik, M.</creator><creator>Sharifah, S.H.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>7U9</scope><scope>H94</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-8499-2152</orcidid></search><sort><creationdate>201510</creationdate><title>Anti‐dengue virus serotype 2 activity and mode of action of a novel peptide</title><author>Chew, M.‐F. ; Tham, H.‐W. ; Rajik, M. ; Sharifah, S.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4911-cbb1b7556e2838c817be018fc6e259ea679caa7ecb6c6feb4eb46bd60a2663a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>antiviral</topic><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>Antiviral drugs</topic><topic>Cercopithecus aethiops</topic><topic>Dengue - virology</topic><topic>Dengue fever</topic><topic>Dengue virus</topic><topic>Dengue Virus - classification</topic><topic>Dengue Virus - drug effects</topic><topic>Dengue Virus - isolation & purification</topic><topic>Dengue Virus - physiology</topic><topic>drug</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>peptide</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Peptides - pharmacology</topic><topic>phage</topic><topic>Pharmacology</topic><topic>Serogroup</topic><topic>Vero Cells</topic><topic>Viral Plaque Assay</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chew, M.‐F.</creatorcontrib><creatorcontrib>Tham, H.‐W.</creatorcontrib><creatorcontrib>Rajik, M.</creatorcontrib><creatorcontrib>Sharifah, S.H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chew, M.‐F.</au><au>Tham, H.‐W.</au><au>Rajik, M.</au><au>Sharifah, S.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti‐dengue virus serotype 2 activity and mode of action of a novel peptide</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2015-10</date><risdate>2015</risdate><volume>119</volume><issue>4</issue><spage>1170</spage><epage>1180</epage><pages>1170-1180</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aim
To identify a novel antiviral peptide against dengue virus serotype 2 (DENV‐2) by screening a phage display peptide library and to evaluate its in vitro antiviral activity and mode of action.
Methods and Results
A phage display peptide library was biopanned against purified DENV‐2 and resulted in the identification and selection of a peptide (peptide gg‐ww) for further investigation. ELISA was performed, and peptide gg‐ww was shown to possess the highest binding affinity against DENV‐2. Thus, peptide gg‐ww was synthesized for cytotoxicity and antiviral assays. Virus plaque reduction assay, real‐time PCR and immunofluorescence assay were used to investigate the inhibitory effect of peptide gg‐ww on DENV‐2 infection in Vero cells. Three different assays (pre‐, simultaneous and post‐treatments assays) were performed to investigate the peptide's mode of action. Results indicated that peptide gg‐ww possessed strong antiviral activity with a ~96% inhibition rate, which was achieved at 250 μmol l−1. Viral replication was inhibited during a simultaneous treatment assay, indicating that the entry of the virus was impeded by this peptide.
Conclusions
Peptide gg‐ww displayed antiviral action against DENV‐2 by targeting an early stage of viral replication (i.e. during viral entry).
Significance and Impact of the Study
Peptide gg‐ww may represent a new therapeutic candidate for the treatment of DENV infections and is a potential candidate to be developed as a peptide drug.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26248692</pmid><doi>10.1111/jam.12921</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8499-2152</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2015-10, Vol.119 (4), p.1170-1180 |
| issn | 1364-5072 1365-2672 |
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| source | Alma/SFX Local Collection |
| subjects | Animals antiviral Antiviral Agents - chemistry Antiviral Agents - pharmacology Antiviral drugs Cercopithecus aethiops Dengue - virology Dengue fever Dengue virus Dengue Virus - classification Dengue Virus - drug effects Dengue Virus - isolation & purification Dengue Virus - physiology drug Humans Molecular Sequence Data peptide Peptides Peptides - chemistry Peptides - pharmacology phage Pharmacology Serogroup Vero Cells Viral Plaque Assay Virus Replication - drug effects |
| title | Anti‐dengue virus serotype 2 activity and mode of action of a novel peptide |
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