The immunomodulatory properties of human bone marrow-derived mesenchymal stromal cells are defined according to multiple immunobiological criteria

Objective Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) are well known to modulate T cells. However, the molecular mechanisms that mark hBM-MSCs immunomodulation of T cells are not fully resolved. Materials and methods hBM-MSCs harvested from sternum or iliac crest of five healthy d...

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Bibliographic Details
Published in:Inflammation research 2016-06, Vol.65 (6), p.501-510
Main Authors: Fayyad-Kazan, Hussein, Faour, Wissam H., Badran, Bassam, Lagneaux, Laurence, Najar, Mehdi
Format: Article
Language:English
Subjects:
Allergology
Apoptosis
Biomedical and Life Sciences
Biomedicine
Bone Marrow
Cell Proliferation
Cell Survival
Cells, Cultured
Coculture Techniques
Dermatology
Humans
Immunology
Immunomodulation
Interleukin-10 - immunology
Interleukin-11 - immunology
Lymphocyte Activation
Mesenchymal Stromal Cells - immunology
Neurology
Original Research Paper
Pharmacology/Toxicology
Rheumatology
T-Lymphocytes - immunology
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Summary:Objective Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) are well known to modulate T cells. However, the molecular mechanisms that mark hBM-MSCs immunomodulation of T cells are not fully resolved. Materials and methods hBM-MSCs harvested from sternum or iliac crest of five healthy donors and characterized in accordance with the International Society of Cellular Therapy (ISCT) guidelines are co-cultured with T cells. Additionally, modulatory effects of MSCs on T-cell viability, proliferation, cytokine profile, co-stimulatory pathway, activation and immunomodulation are also determined. Results hBM-MSCs significantly reduced the expression of T-cell activation marker CD38 as well as co-stimulatory markers CD134 and CD154, whilst that of CD27 remained unchanged. BrdU, CFSE and Ki67 proliferation assays showed that hBM-MSCs reduced T-cell proliferation. Moreover, viability of T cells remained unchanged when co-cultured with hBM-MSCs. Finally, T cells when co-cultured with hBM-MSCs showed increased secretion of IL-10 and IL-11. Conclusion Collectively, hBM-MSCs are able to modulate the main steps involved in T-cell response toward a tolerogenic state. Thus, establishing immunobiological criteria defining the immunosuppressive effect of hBM-MSCs is of importance to reach efficient immunotherapeutic intervention.
ISSN:1023-3830
1420-908X
DOI:10.1007/s00011-016-0933-2