Enhanced biosynthesis of O‐desmethylangolensin from daidzein by a novel oxygen‐tolerant cock intestinal bacterium in the presence of atmospheric oxygen

• Published in: Journal of applied microbiology 2015-03, Vol.118 (3), p.619-628
• Main Authors: Li, M, Li, H, Zhang, C, Wang, X.‐L, Chen, B.‐H, Hao, Q.‐H, Wang, S.‐Y
• Format: Article
• Language: English
• Subjects: absorbance; aerobic biotransformation; Aerobiosis; Animals; Bacteria; bacterial isolation; Biosynthesis; biotransformation; Clostridium; Clostridium - growth & development; Clostridium - isolation & purification; Clostridium - metabolism; daidzein; domestication; Intestines - microbiology; isoflavone daidzein; Isoflavones - biosynthesis; Isoflavones - metabolism; microbial growth; Microbiology; Molecular Sequence Data; nucleotide sequences; Oxygen; oxygen‐tolerant domestication; O‐desmethylangolensin; ribosomal RNA
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12732

AIMS: To improve the oxygen‐tolerant capability of a newly isolated anaerobic bacterium and to biosynthesize O‐desmethylangolensin (O‐Dma) from daidzein aerobically. METHODS AND RESULTS: After a long‐term domestication process, an oxygen‐tolerant bacterium, which we named Aeroto‐AUH‐JLC108, was derived from the newly isolated obligate anaerobic bacterium Clostridium sp. AUH‐JLC108. Strain Aeroto‐AUH‐JLC108 differed from the natively anaerobic wild‐type strain AUH‐JLC108 by various characteristics, including a change in bacterial shape, biochemical characteristics and 16S rRNA gene sequences. Both the growth speed and the maximal optical density (OD) value of strain Aeroto‐AUH‐JLC108 grown aerobically were significantly increased compared to that of the wild‐type strain grown anaerobically. The maximal concentration of the substrate daidzein that the oxygen‐tolerant strain Aeroto‐AUH‐JLC108 grown aerobically was able to convert efficiently was 2·0 mmol l⁻¹and 0·6 mmol l⁻¹for strain AUH‐JLC108 that was grown anaerobically. CONCLUSIONS: Strain Aeroto‐AUH‐JLC108 is a conditional oxygen‐tolerant bacterium. The growth speed, bacterial growth mass and bioconversion capability of strain Aeroto‐AUH‐JLC108 grown aerobically was significantly increased compared to that of the wild‐type strain AUH‐JLC108 grown anaerobically. SIGNIFICANCE AND IMPACT OF THE STUDY: Strain Aeroto‐AUH‐JLC108 is the first reported pure culture responsible for the formation of O‐Dma from daidzein in the presence of atmospheric oxygen.