Microbial Reduction of Fe(III) and Sorption/Precipitation of Fe(II) on Shewanella putrefaciens Strain CN32

• Published in: Environmental science & technology 2001-04, Vol.35 (7), p.1385-1393
• Main Authors: Liu, Chongxuan, Zachara, John M, Gorby, Yuri A, Szecsody, Jim E, Brown, Christopher F
• Format: Article
• Language: English
• Subjects: Adsorption; Animal, plant and microbial ecology; Applied sciences; Biodegradation, Environmental; Biological and medical sciences; Biological and physicochemical phenomena; Biological and physicochemical properties of pollutants. Interaction in the soil; Chemical Precipitation; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Environmental impact; Exact sciences and technology; Ferric Compounds - metabolism; Ferrous Compounds - chemistry; Fundamental and applied biological sciences. Psychology; Iron; Isotopes; Microbial ecology; Microscopy, Electron; Natural water pollution; Oxidation; Oxidation-Reduction; Pollution; Pollution, environment geology; Shewanella putrefaciens; Shewanella putrefaciens - metabolism; Soil; Soil and sediments pollution; Studies; Waste Disposal, Fluid; Water treatment and pollution
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es0015139

The influence of Fe(II) on the dissimilatory bacterial reduction of an Fe(III) aqueous complex (Fe(III)−citrateaq) was investigated using Shewanella putrefaciens strain CN32. The sorption of Fe(II) on CN32 followed a Langmuir isotherm. Least-squares fitting gave a maximum sorption capacity of Q max = 4.19 × 10-3 mol/1012 cells (1.19 mmol/m2 of cell surface area) and an affinity coefficient of log K = 3.29. The growth yield of CN32 with respect to Fe(III)aq reduction showed a linear trend with an average value of 5.24 (±0.12) × 109 cells/mmol of Fe(III). The reduction of Fe(III)aq by CN32 was described by Monod kinetics with respect to the electron acceptor concentration, Fe(III)aq, with a half-saturation constant (K s) of 29 (±3) mM and maximum growth rate (μmax) of 0.32 (±0.02) h-1. However, the pretreatment of CN32 with Fe(II)aq significantly inhibited the reduction of Fe(III)aq, resulting in a lag phase of about 3−30 h depending on initial cell concentrations. Lower initial cell concentration led to longer lag phase duration, and higher cell concentration led to a shorter one. Transmission electron microscopy and energy dispersive spectroscopy revealed that many cells carried surface precipitates of Fe mineral phases (valence unspecified) during the lag phase. These precipitates disappeared after the cells recovered from the lag phase. The cell inhibition and recovery mechanisms from Fe(II)-induced mineral precipitation were not identified by this study, but several alternatives were discussed. A modified Monod model incorporating a lag phase, Fe(II) adsorption, and aqueous complexation reactions was able to describe the experimental results of microbial Fe(III)aq reduction and cell growth when cells were pretreated with Fe(II)aq.