Cross reactivity of different specific Micrurus antivenom sera with homologous and heterologous snake venoms

Coral snakes are the only Elapids in America. They are represented by three genera: Leptomicrurus, Micruroides and Micrurus, of which the latter are the most abundant and diversified group. Little is known about the biochemistry of Micrurus venoms due to low availability. Here, we present a study on...

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Bibliographic Details
Published in:Toxicon (Oxford) 2001-07, Vol.39 (7), p.949-953
Main Authors: DE BRANDAO PRIETO DA SILVA, Alvaro Rossan, YAMAGUSHI, Ivone Kasuko, MORAIS, Josefina Farina, HIGASHI, Hisako Gondo, RAW, Isaias, HO, Paulo Lee, SILVEIRA DE OLIVERIRA, Jane
Format: Article
Language:English
Subjects:
Animal poisons toxicology. Antivenoms
Animals
Antivenins - chemistry
Antivenins - genetics
Antivenins - immunology
Biological and medical sciences
Blotting, Western
Cross Reactions
Elapid Venoms - chemistry
Elapid Venoms - genetics
Elapid Venoms - immunology
Elapidae - metabolism
Electrophoresis, Polyacrylamide Gel
Horses - immunology
Medical sciences
Micrurus
Micrurus corallinus
Neurotoxins - chemistry
Neurotoxins - immunology
Species Specificity
Toxicology
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Summary:Coral snakes are the only Elapids in America. They are represented by three genera: Leptomicrurus, Micruroides and Micrurus, of which the latter are the most abundant and diversified group. Little is known about the biochemistry of Micrurus venoms due to low availability. Here, we present a study on the cross reactivity of different specific Micrurus antivenom with homologous and heterologous snake venoms in order to contribute to the generation of more efficient antiserum for therapeutic purposes. The three specific antisera tested, anti-Micrurus corallinus, anti-Micrurus frontalis, and anti-Micrurus spixii, as well as the bivalent anti-elapid venom sera, raised against a mixture (50% each) of Micrurus frontalis and Micrurus corallinus venoms, were assayed by Western Blot against Micrurus and non-Micrurus elapid venoms. An antisera raised against a recombinant alpha-neurotoxin-like protein from Micrurus corallinus venom, only reacted in Western blot with its homologous venom, indicating that this protein is specific for Micrurus corallinus coral snake.
ISSN:0041-0101
1879-3150
DOI:10.1016/S0041-0101(00)00233-6