Venezuelan kindreds reveal that genetic and environmental factors modulate Huntington's disease age of onset: The U.S.-Venezuela Collaborative Research Project

• Published in: Proceedings of the National Academy of Sciences - PNAS 2004-03, Vol.101 (10), p.3498-3503
• Main Author: Wexler, N S
• Format: Article
• Language: English
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0308679101

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by a triplet (CAG) expansion mutation. The length of the triplet repeat is the most important factor in determining age of onset of HD, although substantial variability remains after controlling for repeat length. The Venezuelan HD kindreds encompass 18,149 individuals spanning 10 generations, 15,409 of whom are living. Of the 4,384 immortalized lymphocyte lines collected, 3,989 DNAs were genotyped for their HD alleles, representing a subset of the population at greatest genetic risk. There are 938 heterozygotes, 80 people with variably penetrant alleles, and 18 homozygotes. Analysis of the 83 kindreds that comprise the Venezuelan HD kindreds demonstrates that residual variability in age of onset has both genetic and environmental components. We created a residual age of onset phenotype from a regression analysis of the log of age of onset on repeat length. Familial correlations (correlation plus or minus SE) were estimated for sibling (0.40 plus or minus 0.09), parent-offspring (0.10 plus or minus 0.11), avuncular (0.07 plus or minus 0.11), and cousin (0.15 plus or minus 0.10) pairs, suggesting a familial origin for the residual variance in onset. By using a variance-components approach with all available familial relationships, the additive genetic heritability of this residual age of onset trait is 38%. A model, including shared sibling environmental effects, estimated the components of additive genetic (0.37), shared environment (0.22), and nonshared environment (0.41) variances, confirming that [approx]40% of the variance remaining in onset age is attributable to genes other than the HD gene and 60% is environmental.