Microsatellite DNA markers for the ground beetle Pterostichus oblongopunctatus F

Six microsatellite DNA loci were isolated from the ground beetle Pterostichus oblongopunctatus F. (Coleoptera, Carabidae) to study population structure. Each locus was polymorphic, with the number of alleles ranging from three to six. Observed heterozygosity varied between 0.20 and 0.67. All six loc...

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Bibliographic Details
Published in:Molecular ecology notes 2004-03, Vol.4 (1), p.113-115
Main Authors: Lagisz, M, Wolff, K
Format: Article
Language:English
Subjects:
alleles
alleles per locus
beetles
Carabidae
Coleoptera
DNA primers
genetic markers
heterozygosity
loci
microsatellite repeats
microsatellites
molecular sequence data
nucleotide sequences
polymerase chain reaction
polymorphic loci
primers
Pterostichus
Pterostichus oblongopunctatus
Pterostichus quadrifoveolatus
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Summary:Six microsatellite DNA loci were isolated from the ground beetle Pterostichus oblongopunctatus F. (Coleoptera, Carabidae) to study population structure. Each locus was polymorphic, with the number of alleles ranging from three to six. Observed heterozygosity varied between 0.20 and 0.67. All six loci were tested for amplification in the closely related P. quadrifoveolatus and they were shown to be polymorphic. The primers developed were used in multiplex polymerase chain reactions.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00586.x