The 4H84 monoclonal antibody detecting beta sub(2)m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes

We have found that 4H84 monoclonal antibody (mAb) used for detection of beta sub(2)m free HLA-G molecules also binds to free heavy chains of classical HLA class I antigens generated on the cell surface by mild acid treatment. Here we demonstrate that beta sub(2)m free classical HLA class I molecules...

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Bibliographic Details
Published in:Human immunology 2004-02, Vol.65 (2), p.157-162
Main Authors: Polakova, K, Kuba, D, Russ, G
Format: Article
Language:English
Online Access:Get full text
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Summary:We have found that 4H84 monoclonal antibody (mAb) used for detection of beta sub(2)m free HLA-G molecules also binds to free heavy chains of classical HLA class I antigens generated on the cell surface by mild acid treatment. Here we demonstrate that beta sub(2)m free classical HLA class I molecules induced on the surface of activated lymphocytes not expressing HLA-G also bind 4H84 mAb. These results demonstrate that 4H84 mAb should be used for detection of HLA-G in cells and tissues with backing by other HLA-G specific mAbs.
ISSN:0198-8859
DOI:10.1016/j.humimm.2003.10.005