2,5-Diketo-D-gluconate production by a mixed culture of two newly-isolated strains : Flavimonas oryzihabitans and Pseudomonas cepacia

A mixed culture of two Gram-negative bacteria isolated from soil converted 50 g d-glucose l^sup -1^ to 2,5-diketo-d-gluconate (2,5 DKG) in 92% yield within 150 h. The first strain, producing 2-keto-d-gluconate (2 KDG) from d-glucose via d-gluconate (DG), was classified as Flavimonas oryzihabitans. T...

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Bibliographic Details
Published in:Biotechnology letters 2001-05, Vol.23 (9), p.693-696
Main Authors: SULO, Pavol, HUDECOVA, Daniela, PROPEROVA, Antonia, BASNAK, Ivan, SEDLACEK, Ivo
Format: Article
Language:English
Subjects:
2,5-Diketo-D-gluconic acid
2-Keto-D-gluconic acid
Bacteria
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Flavimonas oryzihabitans
Fundamental and applied biological sciences. Psychology
gluconic acid
Methods. Procedures. Technologies
Pseudomonas cepacia
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Summary:A mixed culture of two Gram-negative bacteria isolated from soil converted 50 g d-glucose l^sup -1^ to 2,5-diketo-d-gluconate (2,5 DKG) in 92% yield within 150 h. The first strain, producing 2-keto-d-gluconate (2 KDG) from d-glucose via d-gluconate (DG), was classified as Flavimonas oryzihabitans. The second strain, that converts 2 KDG to 2,5 DKG, was identified as Pseudomonas cepacia. This approach presents a new possibility to produce ascorbic acid by microbial transformation, including the use of other, more convenient substrates.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010360603287