Granulocyte Colony-Stimulating Factor Receptors on Granulocytes Are Down-Regulated after Endotoxin Administration to Healthy Humans

Granulocyte colony-stimulating factor (G-CSF) is considered an important mediator of host defense against infection, and recombinant G-CSF is administered to patients with various infections. G-CSF binds to a specific receptor that is expressed on granulocytes and monocytes. To obtain insight about...

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Bibliographic Details
Published in:The Journal of infectious diseases 2000-06, Vol.181 (6), p.2067-2070
Main Authors: Dekkers, Pascale E. P., Juffermans, Nicole P., ten Hove, Tessa, de Jonge, Evert, van Deventer, Sander J. H., van der Poll, Tom
Format: Article
Language:English
Subjects:
Adult
Antibodies
Biological and medical sciences
Blood
Colony stimulating factors
Concise Communications
Fundamental and applied biological sciences. Psychology
Granulocyte colony stimulating factor receptors
Granulocytes
Granulocytes - chemistry
Health care administration
Humans
Infections
Inflammation
Lipopolysaccharides - toxicity
Male
Molecular and cellular biology
Monocytes
Neutrophils
Receptors
Receptors, Granulocyte Colony-Stimulating Factor - analysis
Time Factors
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Summary:Granulocyte colony-stimulating factor (G-CSF) is considered an important mediator of host defense against infection, and recombinant G-CSF is administered to patients with various infections. G-CSF binds to a specific receptor that is expressed on granulocytes and monocytes. To obtain insight about the regulation of the G-CSF receptor after an acute infectious challenge, 8 healthy subjects received an intravenous injection of lipopolysaccharide (LPS; 4 ng/kg), and receptor expression was determined on blood leukocytes by fluorescence-activated cell sorter analysis, both by measurement of saturation binding of recombinant G-CSF and by use of an anti-G-CSF-receptor antibody. LPS induced a transient decrease in granulocyte, but not monocyte, G-CSF-receptor expression. In whole blood in vitro, not only LPS but also gram-positive stimuli and proinflammatory cytokines were capable of down-modulating the G-CSF receptor on granulocytes. Bacterial antigens down-regulate the G-CSF receptor at the surface of granulocytes, which may impair neutrophil functions important for antibacterial host defense.
ISSN:0022-1899
1537-6613
DOI:10.1086/315523