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[11C]acetate and PET/CT assessment of muscle activation in rat studies
Purpose The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [ 11 C ]acetate positron emission tomography and computed tomography (PET/CT). Methods Contractions were induced in Wistar rat...
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| Published in: | International journal for computer assisted radiology and surgery 2016-05, Vol.11 (5), p.733-743 |
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| container_issue | 5 |
| container_start_page | 733 |
| container_title | International journal for computer assisted radiology and surgery |
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| creator | Trombella, Sara García, David Colin, Didier J. Germain, Stéphane Seimbille, Yann Ratib, Osman |
| description | Purpose
The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [
11
C
]acetate positron emission tomography and computed tomography (PET/CT).
Methods
Contractions were induced in Wistar rats’ left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [
11
C
]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters
K
1
and
k
2
were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed (
K
1
E
x
/
K
1
R
e
,
k
2
E
x
/
k
2
R
e
and
SUV
E
x
/
SUV
R
e
, respectively).
Results
Kinetic analysis quantitatively confirmed that net tracer uptake (
K
1
) and washout (
k
2
) were significantly higher in exercised muscles (
K
1
:
0.34
±
0.12
min
-
1
for exercised muscles vs.
0.18
±
0.09
min
-
1
for resting muscles,
P
=
0.01
;
k
2
:
0.22
±
0.05
min
-
1
for exercised muscle vs.
0.14
±
0.04
min
-
1
for resting muscle,
P
=
0.002
). On the other hand, SUV was not significantly different between active and inactive muscles (
0.7
±
0.2
for exercised muscles vs.
0.6
±
0.1
for resting muscles). Linear regression analysis revealed a good correlation (
R
2
=
0.75
,
P
=
0.005
) between net tracer uptake ratio (
K
1
E
x
/
K
1
R
e
) and the SUV ratio
(
SUV
E
x
/
SUV
R
e
). A lower correlation was found between the net tracer washout ratio (
k
2
E
x
/
k
2
R
e
) and the SUV ratio (
R
2
=
0.37
,
P
=
0.1
).
Conclusion
The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [
11
C
]acetate PET/CT. |
| doi_str_mv | 10.1007/s11548-015-1260-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1789038347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1789038347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-406348a211cb46bed31bdeed00aa8b6b97deb0fabc4ec3a50cbbd966cdf404a83</originalsourceid><addsrcrecordid>eNp9kMtKxEAQRRtRfIx-gBvJ0k20Kul0kqUEXzCgi3El0vSjIhnyGFMdwb83w6hLV1VwT12oI8Q5whUC5NeMmMkiBsxiTBTExZ44xkJhrGRS7v_tCEfihHkNILM8zQ7FUaIShFLCsbh7RazejKNgAkWm99Hz7eq6WkWGmZg76kM01FE3sWvn3IXm04Rm6KOmj0YTIg6Tb4hPxUFtWqazn7kQL3e3q-ohXj7dP1Y3y9ilUoZYgkplYRJEZ6Wy5FO0nsgDGFNYZcvck4XaWCfJpSYDZ60vlXK-liBNkS7E5a53Mw4fE3HQXcOO2tb0NEysMS9KSItU5jOKO9SNA_NItd6MTWfGL42gt_r0Tp-e9emtPr2tv_ipn2xH_u_i19cMJDuA56h_p1Gvh2ns55f_af0GnXB6hQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1789038347</pqid></control><display><type>article</type><title>[11C]acetate and PET/CT assessment of muscle activation in rat studies</title><source>Springer Link</source><creator>Trombella, Sara ; García, David ; Colin, Didier J. ; Germain, Stéphane ; Seimbille, Yann ; Ratib, Osman</creator><creatorcontrib>Trombella, Sara ; García, David ; Colin, Didier J. ; Germain, Stéphane ; Seimbille, Yann ; Ratib, Osman</creatorcontrib><description>Purpose
The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [
11
C
]acetate positron emission tomography and computed tomography (PET/CT).
Methods
Contractions were induced in Wistar rats’ left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [
11
C
]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters
K
1
and
k
2
were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed (
K
1
E
x
/
K
1
R
e
,
k
2
E
x
/
k
2
R
e
and
SUV
E
x
/
SUV
R
e
, respectively).
Results
Kinetic analysis quantitatively confirmed that net tracer uptake (
K
1
) and washout (
k
2
) were significantly higher in exercised muscles (
K
1
:
0.34
±
0.12
min
-
1
for exercised muscles vs.
0.18
±
0.09
min
-
1
for resting muscles,
P
=
0.01
;
k
2
:
0.22
±
0.05
min
-
1
for exercised muscle vs.
0.14
±
0.04
min
-
1
for resting muscle,
P
=
0.002
). On the other hand, SUV was not significantly different between active and inactive muscles (
0.7
±
0.2
for exercised muscles vs.
0.6
±
0.1
for resting muscles). Linear regression analysis revealed a good correlation (
R
2
=
0.75
,
P
=
0.005
) between net tracer uptake ratio (
K
1
E
x
/
K
1
R
e
) and the SUV ratio
(
SUV
E
x
/
SUV
R
e
). A lower correlation was found between the net tracer washout ratio (
k
2
E
x
/
k
2
R
e
) and the SUV ratio (
R
2
=
0.37
,
P
=
0.1
).
Conclusion
The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [
11
C
]acetate PET/CT.</description><identifier>ISSN: 1861-6410</identifier><identifier>EISSN: 1861-6429</identifier><identifier>DOI: 10.1007/s11548-015-1260-8</identifier><identifier>PMID: 26210940</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetates ; Animals ; Carbon Radioisotopes ; Computer Imaging ; Computer Science ; Health Informatics ; Hindlimb - blood supply ; Hindlimb - diagnostic imaging ; Hindlimb - metabolism ; Imaging ; Kinetics ; Male ; Medicine ; Medicine & Public Health ; Muscle Contraction - physiology ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - diagnostic imaging ; Muscle, Skeletal - metabolism ; Original Article ; Pattern Recognition and Graphics ; Positron Emission Tomography Computed Tomography ; Radiology ; Rats ; Rats, Wistar ; Surgery ; Vision</subject><ispartof>International journal for computer assisted radiology and surgery, 2016-05, Vol.11 (5), p.733-743</ispartof><rights>CARS 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-406348a211cb46bed31bdeed00aa8b6b97deb0fabc4ec3a50cbbd966cdf404a83</citedby><cites>FETCH-LOGICAL-c344t-406348a211cb46bed31bdeed00aa8b6b97deb0fabc4ec3a50cbbd966cdf404a83</cites><orcidid>0000-0001-6299-2066</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26210940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trombella, Sara</creatorcontrib><creatorcontrib>García, David</creatorcontrib><creatorcontrib>Colin, Didier J.</creatorcontrib><creatorcontrib>Germain, Stéphane</creatorcontrib><creatorcontrib>Seimbille, Yann</creatorcontrib><creatorcontrib>Ratib, Osman</creatorcontrib><title>[11C]acetate and PET/CT assessment of muscle activation in rat studies</title><title>International journal for computer assisted radiology and surgery</title><addtitle>Int J CARS</addtitle><addtitle>Int J Comput Assist Radiol Surg</addtitle><description>Purpose
The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [
11
C
]acetate positron emission tomography and computed tomography (PET/CT).
Methods
Contractions were induced in Wistar rats’ left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [
11
C
]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters
K
1
and
k
2
were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed (
K
1
E
x
/
K
1
R
e
,
k
2
E
x
/
k
2
R
e
and
SUV
E
x
/
SUV
R
e
, respectively).
Results
Kinetic analysis quantitatively confirmed that net tracer uptake (
K
1
) and washout (
k
2
) were significantly higher in exercised muscles (
K
1
:
0.34
±
0.12
min
-
1
for exercised muscles vs.
0.18
±
0.09
min
-
1
for resting muscles,
P
=
0.01
;
k
2
:
0.22
±
0.05
min
-
1
for exercised muscle vs.
0.14
±
0.04
min
-
1
for resting muscle,
P
=
0.002
). On the other hand, SUV was not significantly different between active and inactive muscles (
0.7
±
0.2
for exercised muscles vs.
0.6
±
0.1
for resting muscles). Linear regression analysis revealed a good correlation (
R
2
=
0.75
,
P
=
0.005
) between net tracer uptake ratio (
K
1
E
x
/
K
1
R
e
) and the SUV ratio
(
SUV
E
x
/
SUV
R
e
). A lower correlation was found between the net tracer washout ratio (
k
2
E
x
/
k
2
R
e
) and the SUV ratio (
R
2
=
0.37
,
P
=
0.1
).
Conclusion
The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [
11
C
]acetate PET/CT.</description><subject>Acetates</subject><subject>Animals</subject><subject>Carbon Radioisotopes</subject><subject>Computer Imaging</subject><subject>Computer Science</subject><subject>Health Informatics</subject><subject>Hindlimb - blood supply</subject><subject>Hindlimb - diagnostic imaging</subject><subject>Hindlimb - metabolism</subject><subject>Imaging</subject><subject>Kinetics</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - diagnostic imaging</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Original Article</subject><subject>Pattern Recognition and Graphics</subject><subject>Positron Emission Tomography Computed Tomography</subject><subject>Radiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Surgery</subject><subject>Vision</subject><issn>1861-6410</issn><issn>1861-6429</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKxEAQRRtRfIx-gBvJ0k20Kul0kqUEXzCgi3El0vSjIhnyGFMdwb83w6hLV1VwT12oI8Q5whUC5NeMmMkiBsxiTBTExZ44xkJhrGRS7v_tCEfihHkNILM8zQ7FUaIShFLCsbh7RazejKNgAkWm99Hz7eq6WkWGmZg76kM01FE3sWvn3IXm04Rm6KOmj0YTIg6Tb4hPxUFtWqazn7kQL3e3q-ohXj7dP1Y3y9ilUoZYgkplYRJEZ6Wy5FO0nsgDGFNYZcvck4XaWCfJpSYDZ60vlXK-liBNkS7E5a53Mw4fE3HQXcOO2tb0NEysMS9KSItU5jOKO9SNA_NItd6MTWfGL42gt_r0Tp-e9emtPr2tv_ipn2xH_u_i19cMJDuA56h_p1Gvh2ns55f_af0GnXB6hQ</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Trombella, Sara</creator><creator>García, David</creator><creator>Colin, Didier J.</creator><creator>Germain, Stéphane</creator><creator>Seimbille, Yann</creator><creator>Ratib, Osman</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6299-2066</orcidid></search><sort><creationdate>20160501</creationdate><title>[11C]acetate and PET/CT assessment of muscle activation in rat studies</title><author>Trombella, Sara ; García, David ; Colin, Didier J. ; Germain, Stéphane ; Seimbille, Yann ; Ratib, Osman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-406348a211cb46bed31bdeed00aa8b6b97deb0fabc4ec3a50cbbd966cdf404a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acetates</topic><topic>Animals</topic><topic>Carbon Radioisotopes</topic><topic>Computer Imaging</topic><topic>Computer Science</topic><topic>Health Informatics</topic><topic>Hindlimb - blood supply</topic><topic>Hindlimb - diagnostic imaging</topic><topic>Hindlimb - metabolism</topic><topic>Imaging</topic><topic>Kinetics</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - diagnostic imaging</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Original Article</topic><topic>Pattern Recognition and Graphics</topic><topic>Positron Emission Tomography Computed Tomography</topic><topic>Radiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Surgery</topic><topic>Vision</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trombella, Sara</creatorcontrib><creatorcontrib>García, David</creatorcontrib><creatorcontrib>Colin, Didier J.</creatorcontrib><creatorcontrib>Germain, Stéphane</creatorcontrib><creatorcontrib>Seimbille, Yann</creatorcontrib><creatorcontrib>Ratib, Osman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for computer assisted radiology and surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trombella, Sara</au><au>García, David</au><au>Colin, Didier J.</au><au>Germain, Stéphane</au><au>Seimbille, Yann</au><au>Ratib, Osman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[11C]acetate and PET/CT assessment of muscle activation in rat studies</atitle><jtitle>International journal for computer assisted radiology and surgery</jtitle><stitle>Int J CARS</stitle><addtitle>Int J Comput Assist Radiol Surg</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>11</volume><issue>5</issue><spage>733</spage><epage>743</epage><pages>733-743</pages><issn>1861-6410</issn><eissn>1861-6429</eissn><abstract>Purpose
The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [
11
C
]acetate positron emission tomography and computed tomography (PET/CT).
Methods
Contractions were induced in Wistar rats’ left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [
11
C
]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters
K
1
and
k
2
were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed (
K
1
E
x
/
K
1
R
e
,
k
2
E
x
/
k
2
R
e
and
SUV
E
x
/
SUV
R
e
, respectively).
Results
Kinetic analysis quantitatively confirmed that net tracer uptake (
K
1
) and washout (
k
2
) were significantly higher in exercised muscles (
K
1
:
0.34
±
0.12
min
-
1
for exercised muscles vs.
0.18
±
0.09
min
-
1
for resting muscles,
P
=
0.01
;
k
2
:
0.22
±
0.05
min
-
1
for exercised muscle vs.
0.14
±
0.04
min
-
1
for resting muscle,
P
=
0.002
). On the other hand, SUV was not significantly different between active and inactive muscles (
0.7
±
0.2
for exercised muscles vs.
0.6
±
0.1
for resting muscles). Linear regression analysis revealed a good correlation (
R
2
=
0.75
,
P
=
0.005
) between net tracer uptake ratio (
K
1
E
x
/
K
1
R
e
) and the SUV ratio
(
SUV
E
x
/
SUV
R
e
). A lower correlation was found between the net tracer washout ratio (
k
2
E
x
/
k
2
R
e
) and the SUV ratio (
R
2
=
0.37
,
P
=
0.1
).
Conclusion
The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [
11
C
]acetate PET/CT.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26210940</pmid><doi>10.1007/s11548-015-1260-8</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6299-2066</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1861-6410 |
| ispartof | International journal for computer assisted radiology and surgery, 2016-05, Vol.11 (5), p.733-743 |
| issn | 1861-6410 1861-6429 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1789038347 |
| source | Springer Link |
| subjects | Acetates Animals Carbon Radioisotopes Computer Imaging Computer Science Health Informatics Hindlimb - blood supply Hindlimb - diagnostic imaging Hindlimb - metabolism Imaging Kinetics Male Medicine Medicine & Public Health Muscle Contraction - physiology Muscle, Skeletal - blood supply Muscle, Skeletal - diagnostic imaging Muscle, Skeletal - metabolism Original Article Pattern Recognition and Graphics Positron Emission Tomography Computed Tomography Radiology Rats Rats, Wistar Surgery Vision |
| title | [11C]acetate and PET/CT assessment of muscle activation in rat studies |
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