Zika virus impairs growth in human neurospheres and brain organoids

Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurosp...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2016-05, Vol.352 (6287), p.816-818
Main Authors: Garcez, Patricia P., Loiola, Erick Correia, da Costa, Rodrigo Madeiro, Higa, Luiza M., Trindade, Pablo, Delvecchio, Rodrigo, Nascimento, Juliana Minardi, Brindeiro, Rodrigo, Tanuri, Amilcar, Rehen, Stevens K.
Format: Article
Language:English
Subjects:
Anatomy
Brain
Brain - abnormalities
Brain - virology
Brazil
Cell Death
Cells, Cultured
Humans
Microcephaly - pathology
Microcephaly - virology
Mosquitoes
Neonates
Neural Stem Cells - pathology
Neural Stem Cells - virology
Neurogenesis
Organoids - abnormalities
Organoids - virology
Pregnancy
Zika virus
Zika Virus - pathogenicity
Zika Virus Infection - complications
Zika Virus Infection - pathology
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Summary:Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurospheres and brain organoids. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aaf6116